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Wide-field fluorescence lifetime imaging of cancer

Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imag...

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Autores principales: McGinty, James, Galletly, Neil P., Dunsby, Chris, Munro, Ian, Elson, Daniel S., Requejo-Isidro, Jose, Cohen, Patrizia, Ahmad, Raida, Forsyth, Amanda, Thillainayagam, Andrew V., Neil, Mark A. A., French, Paul M. W., Stamp, Gordon W
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017991/
https://www.ncbi.nlm.nih.gov/pubmed/21258496
http://dx.doi.org/10.1364/BOE.1.000627
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author McGinty, James
Galletly, Neil P.
Dunsby, Chris
Munro, Ian
Elson, Daniel S.
Requejo-Isidro, Jose
Cohen, Patrizia
Ahmad, Raida
Forsyth, Amanda
Thillainayagam, Andrew V.
Neil, Mark A. A.
French, Paul M. W.
Stamp, Gordon W
author_facet McGinty, James
Galletly, Neil P.
Dunsby, Chris
Munro, Ian
Elson, Daniel S.
Requejo-Isidro, Jose
Cohen, Patrizia
Ahmad, Raida
Forsyth, Amanda
Thillainayagam, Andrew V.
Neil, Mark A. A.
French, Paul M. W.
Stamp, Gordon W
author_sort McGinty, James
collection PubMed
description Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imaging does not always provide sufficient contrast. We demonstrate that fluorescence lifetime imaging (FLIM) applied to intrinsic tissue autofluorescence can directly contrast a range of surface tissue tumors, including in gastrointestinal tissues, using compact, clinically deployable instrumentation achieving wide-field fluorescence lifetime images of unprecedented clarity. Statistically significant contrast is observed between cancerous and healthy colon tissue for FLIM with excitation at 355 nm. To illustrate the clinical potential, wide-field fluorescence lifetime images of unstained ex vivo tissue have been acquired at near video rate, which is an important step towards real-time FLIM for diagnostic and interoperative imaging, including for screening and image-guided biopsy applications.
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spelling pubmed-30179912011-01-21 Wide-field fluorescence lifetime imaging of cancer McGinty, James Galletly, Neil P. Dunsby, Chris Munro, Ian Elson, Daniel S. Requejo-Isidro, Jose Cohen, Patrizia Ahmad, Raida Forsyth, Amanda Thillainayagam, Andrew V. Neil, Mark A. A. French, Paul M. W. Stamp, Gordon W Biomed Opt Express Spectroscopic Diagnostics Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imaging does not always provide sufficient contrast. We demonstrate that fluorescence lifetime imaging (FLIM) applied to intrinsic tissue autofluorescence can directly contrast a range of surface tissue tumors, including in gastrointestinal tissues, using compact, clinically deployable instrumentation achieving wide-field fluorescence lifetime images of unprecedented clarity. Statistically significant contrast is observed between cancerous and healthy colon tissue for FLIM with excitation at 355 nm. To illustrate the clinical potential, wide-field fluorescence lifetime images of unstained ex vivo tissue have been acquired at near video rate, which is an important step towards real-time FLIM for diagnostic and interoperative imaging, including for screening and image-guided biopsy applications. Optical Society of America 2010-08-19 /pmc/articles/PMC3017991/ /pubmed/21258496 http://dx.doi.org/10.1364/BOE.1.000627 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Spectroscopic Diagnostics
McGinty, James
Galletly, Neil P.
Dunsby, Chris
Munro, Ian
Elson, Daniel S.
Requejo-Isidro, Jose
Cohen, Patrizia
Ahmad, Raida
Forsyth, Amanda
Thillainayagam, Andrew V.
Neil, Mark A. A.
French, Paul M. W.
Stamp, Gordon W
Wide-field fluorescence lifetime imaging of cancer
title Wide-field fluorescence lifetime imaging of cancer
title_full Wide-field fluorescence lifetime imaging of cancer
title_fullStr Wide-field fluorescence lifetime imaging of cancer
title_full_unstemmed Wide-field fluorescence lifetime imaging of cancer
title_short Wide-field fluorescence lifetime imaging of cancer
title_sort wide-field fluorescence lifetime imaging of cancer
topic Spectroscopic Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017991/
https://www.ncbi.nlm.nih.gov/pubmed/21258496
http://dx.doi.org/10.1364/BOE.1.000627
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