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Wide-field fluorescence lifetime imaging of cancer
Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imag...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017991/ https://www.ncbi.nlm.nih.gov/pubmed/21258496 http://dx.doi.org/10.1364/BOE.1.000627 |
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author | McGinty, James Galletly, Neil P. Dunsby, Chris Munro, Ian Elson, Daniel S. Requejo-Isidro, Jose Cohen, Patrizia Ahmad, Raida Forsyth, Amanda Thillainayagam, Andrew V. Neil, Mark A. A. French, Paul M. W. Stamp, Gordon W |
author_facet | McGinty, James Galletly, Neil P. Dunsby, Chris Munro, Ian Elson, Daniel S. Requejo-Isidro, Jose Cohen, Patrizia Ahmad, Raida Forsyth, Amanda Thillainayagam, Andrew V. Neil, Mark A. A. French, Paul M. W. Stamp, Gordon W |
author_sort | McGinty, James |
collection | PubMed |
description | Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imaging does not always provide sufficient contrast. We demonstrate that fluorescence lifetime imaging (FLIM) applied to intrinsic tissue autofluorescence can directly contrast a range of surface tissue tumors, including in gastrointestinal tissues, using compact, clinically deployable instrumentation achieving wide-field fluorescence lifetime images of unprecedented clarity. Statistically significant contrast is observed between cancerous and healthy colon tissue for FLIM with excitation at 355 nm. To illustrate the clinical potential, wide-field fluorescence lifetime images of unstained ex vivo tissue have been acquired at near video rate, which is an important step towards real-time FLIM for diagnostic and interoperative imaging, including for screening and image-guided biopsy applications. |
format | Text |
id | pubmed-3017991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30179912011-01-21 Wide-field fluorescence lifetime imaging of cancer McGinty, James Galletly, Neil P. Dunsby, Chris Munro, Ian Elson, Daniel S. Requejo-Isidro, Jose Cohen, Patrizia Ahmad, Raida Forsyth, Amanda Thillainayagam, Andrew V. Neil, Mark A. A. French, Paul M. W. Stamp, Gordon W Biomed Opt Express Spectroscopic Diagnostics Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imaging does not always provide sufficient contrast. We demonstrate that fluorescence lifetime imaging (FLIM) applied to intrinsic tissue autofluorescence can directly contrast a range of surface tissue tumors, including in gastrointestinal tissues, using compact, clinically deployable instrumentation achieving wide-field fluorescence lifetime images of unprecedented clarity. Statistically significant contrast is observed between cancerous and healthy colon tissue for FLIM with excitation at 355 nm. To illustrate the clinical potential, wide-field fluorescence lifetime images of unstained ex vivo tissue have been acquired at near video rate, which is an important step towards real-time FLIM for diagnostic and interoperative imaging, including for screening and image-guided biopsy applications. Optical Society of America 2010-08-19 /pmc/articles/PMC3017991/ /pubmed/21258496 http://dx.doi.org/10.1364/BOE.1.000627 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Spectroscopic Diagnostics McGinty, James Galletly, Neil P. Dunsby, Chris Munro, Ian Elson, Daniel S. Requejo-Isidro, Jose Cohen, Patrizia Ahmad, Raida Forsyth, Amanda Thillainayagam, Andrew V. Neil, Mark A. A. French, Paul M. W. Stamp, Gordon W Wide-field fluorescence lifetime imaging of cancer |
title | Wide-field fluorescence lifetime imaging of cancer |
title_full | Wide-field fluorescence lifetime imaging of cancer |
title_fullStr | Wide-field fluorescence lifetime imaging of cancer |
title_full_unstemmed | Wide-field fluorescence lifetime imaging of cancer |
title_short | Wide-field fluorescence lifetime imaging of cancer |
title_sort | wide-field fluorescence lifetime imaging of cancer |
topic | Spectroscopic Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017991/ https://www.ncbi.nlm.nih.gov/pubmed/21258496 http://dx.doi.org/10.1364/BOE.1.000627 |
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