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Ultrahigh resolution photoacoustic microscopy via transient absorption
We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is func...
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Formato: | Texto |
Lenguaje: | English |
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Optical Society of America
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017994/ https://www.ncbi.nlm.nih.gov/pubmed/21258499 http://dx.doi.org/10.1364/BOE.1.000676 |
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author | Shelton, Ryan L. Applegate, Brian E. |
author_facet | Shelton, Ryan L. Applegate, Brian E. |
author_sort | Shelton, Ryan L. |
collection | PubMed |
description | We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is functionally equivalent to confocal and two-photon fluorescence microscopy, potentially enabling cellular/subcellular photoacoustic imaging. A prototype TAUM system was designed, built, and used to image a cross-section through several capillaries in the excised cheek pouch of a Syrian Hamster. The well-resolved capillaries in the TAUM image provided experimental evidence of the spatial resolution. These results suggest that TAUM has excellent potential for producing volumetric images with cellular/subcellular resolution in three dimensions deep inside living tissue. |
format | Text |
id | pubmed-3017994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30179942011-01-21 Ultrahigh resolution photoacoustic microscopy via transient absorption Shelton, Ryan L. Applegate, Brian E. Biomed Opt Express Photoacoustic Imaging and Spectroscopy We have developed a novel, hybrid imaging modality, Transient Absorption Ultrasonic Microscopy (TAUM), which takes advantage of the optical nonlinearities afforded by transient absorption to achieve ultrahigh-resolution photoacoustic microscopy. The theoretical point spread function for TAUM is functionally equivalent to confocal and two-photon fluorescence microscopy, potentially enabling cellular/subcellular photoacoustic imaging. A prototype TAUM system was designed, built, and used to image a cross-section through several capillaries in the excised cheek pouch of a Syrian Hamster. The well-resolved capillaries in the TAUM image provided experimental evidence of the spatial resolution. These results suggest that TAUM has excellent potential for producing volumetric images with cellular/subcellular resolution in three dimensions deep inside living tissue. Optical Society of America 2010-08-23 /pmc/articles/PMC3017994/ /pubmed/21258499 http://dx.doi.org/10.1364/BOE.1.000676 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Photoacoustic Imaging and Spectroscopy Shelton, Ryan L. Applegate, Brian E. Ultrahigh resolution photoacoustic microscopy via transient absorption |
title | Ultrahigh resolution photoacoustic microscopy via transient absorption |
title_full | Ultrahigh resolution photoacoustic microscopy via transient absorption |
title_fullStr | Ultrahigh resolution photoacoustic microscopy via transient absorption |
title_full_unstemmed | Ultrahigh resolution photoacoustic microscopy via transient absorption |
title_short | Ultrahigh resolution photoacoustic microscopy via transient absorption |
title_sort | ultrahigh resolution photoacoustic microscopy via transient absorption |
topic | Photoacoustic Imaging and Spectroscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017994/ https://www.ncbi.nlm.nih.gov/pubmed/21258499 http://dx.doi.org/10.1364/BOE.1.000676 |
work_keys_str_mv | AT sheltonryanl ultrahighresolutionphotoacousticmicroscopyviatransientabsorption AT applegatebriane ultrahighresolutionphotoacousticmicroscopyviatransientabsorption |