Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy

We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion...

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Autores principales: Isobe, Keisuke, Hashimoto, Hiroshi, Suda, Akira, Kannari, Fumihiko, Kawano, Hiroyuki, Mizuno, Hideaki, Miyawaki, Atsushi, Midorikawa, Katsumi
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Molecular Imaging and Probe Development
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017996/
https://www.ncbi.nlm.nih.gov/pubmed/21258500
http://dx.doi.org/10.1364/BOE.1.000687
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author Isobe, Keisuke
Hashimoto, Hiroshi
Suda, Akira
Kannari, Fumihiko
Kawano, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi
author_facet Isobe, Keisuke
Hashimoto, Hiroshi
Suda, Akira
Kannari, Fumihiko
Kawano, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi
author_sort Isobe, Keisuke
collection PubMed
description We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form.
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spelling pubmed-30179962011-01-21 Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy Isobe, Keisuke Hashimoto, Hiroshi Suda, Akira Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi Biomed Opt Express Molecular Imaging and Probe Development We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form. Optical Society of America 2010-08-23 /pmc/articles/PMC3017996/ /pubmed/21258500 http://dx.doi.org/10.1364/BOE.1.000687 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Molecular Imaging and Probe Development
Isobe, Keisuke
Hashimoto, Hiroshi
Suda, Akira
Kannari, Fumihiko
Kawano, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi
Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title_full Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title_fullStr Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title_full_unstemmed Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title_short Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
title_sort measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (kaede) by nonlinear fourier-transform spectroscopy
topic Molecular Imaging and Probe Development
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017996/
https://www.ncbi.nlm.nih.gov/pubmed/21258500
http://dx.doi.org/10.1364/BOE.1.000687
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