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Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy
We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017996/ https://www.ncbi.nlm.nih.gov/pubmed/21258500 http://dx.doi.org/10.1364/BOE.1.000687 |
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author | Isobe, Keisuke Hashimoto, Hiroshi Suda, Akira Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi |
author_facet | Isobe, Keisuke Hashimoto, Hiroshi Suda, Akira Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi |
author_sort | Isobe, Keisuke |
collection | PubMed |
description | We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form. |
format | Text |
id | pubmed-3017996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30179962011-01-21 Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy Isobe, Keisuke Hashimoto, Hiroshi Suda, Akira Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi Biomed Opt Express Molecular Imaging and Probe Development We demonstrate the measurement of two-photon excitation (TPE) spectra, used not only for fluorescence but also for photoconversion in green-to-red photoconvertible Kaede, using nonlinear Fourier-transform spectroscopy. It was found that in unphotoconverted Kaede, the TPE spectrum for photoconversion is much different to that for green-fluorescence. This is similar to the difference between the one-photon excitation of photoconversion in the neutral form and that of green-fluorescence in the ionized form. Optical Society of America 2010-08-23 /pmc/articles/PMC3017996/ /pubmed/21258500 http://dx.doi.org/10.1364/BOE.1.000687 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Molecular Imaging and Probe Development Isobe, Keisuke Hashimoto, Hiroshi Suda, Akira Kannari, Fumihiko Kawano, Hiroyuki Mizuno, Hideaki Miyawaki, Atsushi Midorikawa, Katsumi Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title | Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title_full | Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title_fullStr | Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title_full_unstemmed | Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title_short | Measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (Kaede) by nonlinear Fourier-transform spectroscopy |
title_sort | measurement of two-photon excitation spectrum used to photoconvert a fluorescent protein (kaede) by nonlinear fourier-transform spectroscopy |
topic | Molecular Imaging and Probe Development |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017996/ https://www.ncbi.nlm.nih.gov/pubmed/21258500 http://dx.doi.org/10.1364/BOE.1.000687 |
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