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Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters
We demonstrate an optical Fourier filtering method which can be used to characterize subcellular morphology during dynamic cellular function. In this paper, our Fourier filters were based on two-dimensional Gabor elementary functions, which can be tuned to sense directly object size and orientation....
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018000/ https://www.ncbi.nlm.nih.gov/pubmed/21258503 http://dx.doi.org/10.1364/BOE.1.000720 |
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author | Pasternack, Robert M. Rabin, Bryan Zheng, Jing-Yi Boustany, Nada N. |
author_facet | Pasternack, Robert M. Rabin, Bryan Zheng, Jing-Yi Boustany, Nada N. |
author_sort | Pasternack, Robert M. |
collection | PubMed |
description | We demonstrate an optical Fourier filtering method which can be used to characterize subcellular morphology during dynamic cellular function. In this paper, our Fourier filters were based on two-dimensional Gabor elementary functions, which can be tuned to sense directly object size and orientation. We utilize this method to quantify changes in mitochondrial and nuclear structure during the first three hours of apoptosis. We find that the technique is sensitive to a decrease in particle orientation consistent with apoptosis-induced mitochondrial fragmentation. The scattering signal changes were less pronounced in the nucleus and the remainder of the cytoplasm. Particles in these regions were less oriented than mitochondria and did not change orientation significantly. |
format | Text |
id | pubmed-3018000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30180002011-01-21 Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters Pasternack, Robert M. Rabin, Bryan Zheng, Jing-Yi Boustany, Nada N. Biomed Opt Express Cell Studies We demonstrate an optical Fourier filtering method which can be used to characterize subcellular morphology during dynamic cellular function. In this paper, our Fourier filters were based on two-dimensional Gabor elementary functions, which can be tuned to sense directly object size and orientation. We utilize this method to quantify changes in mitochondrial and nuclear structure during the first three hours of apoptosis. We find that the technique is sensitive to a decrease in particle orientation consistent with apoptosis-induced mitochondrial fragmentation. The scattering signal changes were less pronounced in the nucleus and the remainder of the cytoplasm. Particles in these regions were less oriented than mitochondria and did not change orientation significantly. Optical Society of America 2010-08-25 /pmc/articles/PMC3018000/ /pubmed/21258503 http://dx.doi.org/10.1364/BOE.1.000720 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Cell Studies Pasternack, Robert M. Rabin, Bryan Zheng, Jing-Yi Boustany, Nada N. Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title | Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title_full | Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title_fullStr | Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title_full_unstemmed | Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title_short | Quantifying subcellular dynamics in apoptotic cells with two-dimensional Gabor filters |
title_sort | quantifying subcellular dynamics in apoptotic cells with two-dimensional gabor filters |
topic | Cell Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018000/ https://www.ncbi.nlm.nih.gov/pubmed/21258503 http://dx.doi.org/10.1364/BOE.1.000720 |
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