Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation

We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viabilit...

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Detalles Bibliográficos
Autores principales: Antkowiak, Maciej, Torres-Mapa, Maria Leilani, Dholakia, Kishan, Gunn-Moore, Frank J.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Cell Studies
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018012/
https://www.ncbi.nlm.nih.gov/pubmed/21258476
http://dx.doi.org/10.1364/BOE.1.000414
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author Antkowiak, Maciej
Torres-Mapa, Maria Leilani
Dholakia, Kishan
Gunn-Moore, Frank J.
author_facet Antkowiak, Maciej
Torres-Mapa, Maria Leilani
Dholakia, Kishan
Gunn-Moore, Frank J.
author_sort Antkowiak, Maciej
collection PubMed
description We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles.
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spelling pubmed-30180122011-01-21 Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation Antkowiak, Maciej Torres-Mapa, Maria Leilani Dholakia, Kishan Gunn-Moore, Frank J. Biomed Opt Express Cell Studies We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles. Optical Society of America 2010-08-02 /pmc/articles/PMC3018012/ /pubmed/21258476 http://dx.doi.org/10.1364/BOE.1.000414 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Cell Studies
Antkowiak, Maciej
Torres-Mapa, Maria Leilani
Dholakia, Kishan
Gunn-Moore, Frank J.
Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title_full Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title_fullStr Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title_full_unstemmed Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title_short Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
title_sort quantitative phase study of the dynamic cellular response in femtosecond laser photoporation
topic Cell Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018012/
https://www.ncbi.nlm.nih.gov/pubmed/21258476
http://dx.doi.org/10.1364/BOE.1.000414
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AT torresmapamarialeilani quantitativephasestudyofthedynamiccellularresponseinfemtosecondlaserphotoporation
AT dholakiakishan quantitativephasestudyofthedynamiccellularresponseinfemtosecondlaserphotoporation
AT gunnmoorefrankj quantitativephasestudyofthedynamiccellularresponseinfemtosecondlaserphotoporation

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