Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues

ABSTRACT: A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz repetition frequency, fiber-probe assemblies, emission monochromator,...

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Detalles Bibliográficos
Autores principales: Lloyd, William R., Wilson, Robert H., Chang, Ching-Wei, Gillispie, Gregory D., Mycek, Mary-Ann
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Spectroscopic Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018017/
https://www.ncbi.nlm.nih.gov/pubmed/21258491
http://dx.doi.org/10.1364/BOE.1.000574
Descripción
Sumario:ABSTRACT: A fiber-optic system was developed to rapidly acquire tissue fluorescence wavelength-time matrices (WTMs) with high signal-to-noise ratio (SNR). The essential system components (473 nm microchip laser operating at 3 kHz repetition frequency, fiber-probe assemblies, emission monochromator, photomultiplier tube, and digitizer) were assembled into a compact and clinically-compatible unit. Data were acquired from fluorescence standards and tissue-simulating phantoms to test system performance. Fluorescence decay waveforms with SNR > 100 at the decay curve peak were obtained in less than 30 ms. With optimized data transfer and monochromator stepping functions, it should be feasible to acquire a full WTM at 5 nm emission wavelength intervals over a 200 nm range in under 2 seconds.

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