Multimodal CARS microscopy of structured carbohydrate biopolymers

We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in...

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Detalles Bibliográficos
Autores principales: Slepkov, Aaron D., Ridsdale, Andrew, Pegoraro, Adrian F., Moffatt, Douglas J., Stolow, Albert
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018121/
https://www.ncbi.nlm.nih.gov/pubmed/21258555
http://dx.doi.org/10.1364/BOE.1.001347
Descripción
Sumario:We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(−1)–3400 cm(−1)), together with the commonly-measured C-H stretch (2750 cm(−1)–2970 cm(−1)) and SHG provide potentially important structural information and contrast in these materials.

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