Multimodal CARS microscopy of structured carbohydrate biopolymers

We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in...

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Autores principales: Slepkov, Aaron D., Ridsdale, Andrew, Pegoraro, Adrian F., Moffatt, Douglas J., Stolow, Albert
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018121/
https://www.ncbi.nlm.nih.gov/pubmed/21258555
http://dx.doi.org/10.1364/BOE.1.001347
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author Slepkov, Aaron D.
Ridsdale, Andrew
Pegoraro, Adrian F.
Moffatt, Douglas J.
Stolow, Albert
author_facet Slepkov, Aaron D.
Ridsdale, Andrew
Pegoraro, Adrian F.
Moffatt, Douglas J.
Stolow, Albert
author_sort Slepkov, Aaron D.
collection PubMed
description We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(−1)–3400 cm(−1)), together with the commonly-measured C-H stretch (2750 cm(−1)–2970 cm(−1)) and SHG provide potentially important structural information and contrast in these materials.
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spelling pubmed-30181212011-01-21 Multimodal CARS microscopy of structured carbohydrate biopolymers Slepkov, Aaron D. Ridsdale, Andrew Pegoraro, Adrian F. Moffatt, Douglas J. Stolow, Albert Biomed Opt Express Microscopy We demonstrate the utility of multimodal coherent anti-Stokes Raman scattering (CARS) microscopy for the study of structured condensed carbohydrate systems. Simultaneous second-harmonic generation (SHG) and spectrally-scanned CARS microscopy was used to elucidate structure, alignment, and density in cellulose cotton fibers and in starch grains undergoing rapid heat-moisture swelling. Our results suggest that CARS response of the O-H stretch region (3000 cm(−1)–3400 cm(−1)), together with the commonly-measured C-H stretch (2750 cm(−1)–2970 cm(−1)) and SHG provide potentially important structural information and contrast in these materials. Optical Society of America 2010-11-08 /pmc/articles/PMC3018121/ /pubmed/21258555 http://dx.doi.org/10.1364/BOE.1.001347 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Microscopy
Slepkov, Aaron D.
Ridsdale, Andrew
Pegoraro, Adrian F.
Moffatt, Douglas J.
Stolow, Albert
Multimodal CARS microscopy of structured carbohydrate biopolymers
title Multimodal CARS microscopy of structured carbohydrate biopolymers
title_full Multimodal CARS microscopy of structured carbohydrate biopolymers
title_fullStr Multimodal CARS microscopy of structured carbohydrate biopolymers
title_full_unstemmed Multimodal CARS microscopy of structured carbohydrate biopolymers
title_short Multimodal CARS microscopy of structured carbohydrate biopolymers
title_sort multimodal cars microscopy of structured carbohydrate biopolymers
topic Microscopy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018121/
https://www.ncbi.nlm.nih.gov/pubmed/21258555
http://dx.doi.org/10.1364/BOE.1.001347
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AT pegoraroadrianf multimodalcarsmicroscopyofstructuredcarbohydratebiopolymers
AT moffattdouglasj multimodalcarsmicroscopyofstructuredcarbohydratebiopolymers
AT stolowalbert multimodalcarsmicroscopyofstructuredcarbohydratebiopolymers

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