Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the availabl...

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Detalles Bibliográficos
Autores principales: Grønlund, Mette, Olsen, Anne, Johansen, Elisabeth I, Jakobsen, Iver
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018388/
https://www.ncbi.nlm.nih.gov/pubmed/21156044
http://dx.doi.org/10.1186/1746-4811-6-28
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author Grønlund, Mette
Olsen, Anne
Johansen, Elisabeth I
Jakobsen, Iver
author_facet Grønlund, Mette
Olsen, Anne
Johansen, Elisabeth I
Jakobsen, Iver
author_sort Grønlund, Mette
collection PubMed
description Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF). Here we describe a PEBV-VIGS protocol suitable for reverse genetics studies in pea of genes involved in the symbiosis with AMF and show its effectiveness in silencing genes involved in the early and late stages of AMF symbiosis.
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spelling pubmed-30183882011-01-11 Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum Grønlund, Mette Olsen, Anne Johansen, Elisabeth I Jakobsen, Iver Plant Methods Methodology Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF). Here we describe a PEBV-VIGS protocol suitable for reverse genetics studies in pea of genes involved in the symbiosis with AMF and show its effectiveness in silencing genes involved in the early and late stages of AMF symbiosis. BioMed Central 2010-12-14 /pmc/articles/PMC3018388/ /pubmed/21156044 http://dx.doi.org/10.1186/1746-4811-6-28 Text en Copyright ©2010 Grønlund et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Grønlund, Mette
Olsen, Anne
Johansen, Elisabeth I
Jakobsen, Iver
Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title_full Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title_fullStr Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title_full_unstemmed Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title_short Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
title_sort protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in pisum sativum
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018388/
https://www.ncbi.nlm.nih.gov/pubmed/21156044
http://dx.doi.org/10.1186/1746-4811-6-28
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