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Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells
BACKGROUND: Hypoxia plays a critical role in various cellular mechanisms, including proliferation and differentiation of neural stem and progenitor cells. In the present study, we explored the impact of lowered oxygen on the differentiation potential of human neural progenitor cells, and the role of...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018408/ https://www.ncbi.nlm.nih.gov/pubmed/21126346 http://dx.doi.org/10.1186/1471-2121-11-94 |
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author | Giese, Anne-Katrin Frahm, Jana Hübner, Rayk Luo, Jiankai Wree, Andreas Frech, Moritz J Rolfs, Arndt Ortinau, Stefanie |
author_facet | Giese, Anne-Katrin Frahm, Jana Hübner, Rayk Luo, Jiankai Wree, Andreas Frech, Moritz J Rolfs, Arndt Ortinau, Stefanie |
author_sort | Giese, Anne-Katrin |
collection | PubMed |
description | BACKGROUND: Hypoxia plays a critical role in various cellular mechanisms, including proliferation and differentiation of neural stem and progenitor cells. In the present study, we explored the impact of lowered oxygen on the differentiation potential of human neural progenitor cells, and the role of erythropoietin in the differentiation process. RESULTS: In this study we demonstrate that differentiation of human fetal neural progenitor cells under hypoxic conditions results in an increased neurogenesis. In addition, expansion and proliferation under lowered oxygen conditions also increased neuronal differentiation, although proliferation rates were not altered compared to normoxic conditions. Erythropoietin partially mimicked these hypoxic effects, as shown by an increase of the metabolic activity during differentiation and protection of differentiated cells from apoptosis. CONCLUSION: These results provide evidence that hypoxia promotes the differentiation of human fetal neural progenitor cells, and identifies the involvement of erythropoietin during differentiation as well as different cellular mechanisms underlying the induction of differentiation mediated by lowered oxygen levels. |
format | Text |
id | pubmed-3018408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30184082011-01-11 Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells Giese, Anne-Katrin Frahm, Jana Hübner, Rayk Luo, Jiankai Wree, Andreas Frech, Moritz J Rolfs, Arndt Ortinau, Stefanie BMC Cell Biol Research Article BACKGROUND: Hypoxia plays a critical role in various cellular mechanisms, including proliferation and differentiation of neural stem and progenitor cells. In the present study, we explored the impact of lowered oxygen on the differentiation potential of human neural progenitor cells, and the role of erythropoietin in the differentiation process. RESULTS: In this study we demonstrate that differentiation of human fetal neural progenitor cells under hypoxic conditions results in an increased neurogenesis. In addition, expansion and proliferation under lowered oxygen conditions also increased neuronal differentiation, although proliferation rates were not altered compared to normoxic conditions. Erythropoietin partially mimicked these hypoxic effects, as shown by an increase of the metabolic activity during differentiation and protection of differentiated cells from apoptosis. CONCLUSION: These results provide evidence that hypoxia promotes the differentiation of human fetal neural progenitor cells, and identifies the involvement of erythropoietin during differentiation as well as different cellular mechanisms underlying the induction of differentiation mediated by lowered oxygen levels. BioMed Central 2010-12-02 /pmc/articles/PMC3018408/ /pubmed/21126346 http://dx.doi.org/10.1186/1471-2121-11-94 Text en Copyright ©2010 Giese et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Giese, Anne-Katrin Frahm, Jana Hübner, Rayk Luo, Jiankai Wree, Andreas Frech, Moritz J Rolfs, Arndt Ortinau, Stefanie Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title | Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title_full | Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title_fullStr | Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title_full_unstemmed | Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title_short | Erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
title_sort | erythropoietin and the effect of oxygen during proliferation and differentiation of human neural progenitor cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018408/ https://www.ncbi.nlm.nih.gov/pubmed/21126346 http://dx.doi.org/10.1186/1471-2121-11-94 |
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