Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and...

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Autores principales: Sander, Jeffry D., Dahlborg, Elizabeth J., Goodwin, Mathew J., Cade, Lindsay, Zhang, Feng, Cifuentes, Daniel, Curtin, Shaun J., Blackburn, Jessica S., Thibodeau-Beganny, Stacey, Qi, Yiping, Pierick, Christopher J., Hoffman, Ellen, Maeder, Morgan L., Khayter, Cyd, Reyon, Deepak, Dobbs, Drena, Langenau, David M., Stupar, Robert M., Giraldez, Antonio J., Voytas, Daniel F., Peterson, Randall T., Yeh, Jing-Ruey J., Joung, J. Keith
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018472/
https://www.ncbi.nlm.nih.gov/pubmed/21151135
http://dx.doi.org/10.1038/nmeth.1542
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author Sander, Jeffry D.
Dahlborg, Elizabeth J.
Goodwin, Mathew J.
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun J.
Blackburn, Jessica S.
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher J.
Hoffman, Ellen
Maeder, Morgan L.
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David M.
Stupar, Robert M.
Giraldez, Antonio J.
Voytas, Daniel F.
Peterson, Randall T.
Yeh, Jing-Ruey J.
Joung, J. Keith
author_facet Sander, Jeffry D.
Dahlborg, Elizabeth J.
Goodwin, Mathew J.
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun J.
Blackburn, Jessica S.
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher J.
Hoffman, Ellen
Maeder, Morgan L.
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David M.
Stupar, Robert M.
Giraldez, Antonio J.
Voytas, Daniel F.
Peterson, Randall T.
Yeh, Jing-Ruey J.
Joung, J. Keith
author_sort Sander, Jeffry D.
collection PubMed
description Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.
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spelling pubmed-30184722011-07-01 Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA) Sander, Jeffry D. Dahlborg, Elizabeth J. Goodwin, Mathew J. Cade, Lindsay Zhang, Feng Cifuentes, Daniel Curtin, Shaun J. Blackburn, Jessica S. Thibodeau-Beganny, Stacey Qi, Yiping Pierick, Christopher J. Hoffman, Ellen Maeder, Morgan L. Khayter, Cyd Reyon, Deepak Dobbs, Drena Langenau, David M. Stupar, Robert M. Giraldez, Antonio J. Voytas, Daniel F. Peterson, Randall T. Yeh, Jing-Ruey J. Joung, J. Keith Nat Methods Article Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations. 2010-12-12 2011-01 /pmc/articles/PMC3018472/ /pubmed/21151135 http://dx.doi.org/10.1038/nmeth.1542 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sander, Jeffry D.
Dahlborg, Elizabeth J.
Goodwin, Mathew J.
Cade, Lindsay
Zhang, Feng
Cifuentes, Daniel
Curtin, Shaun J.
Blackburn, Jessica S.
Thibodeau-Beganny, Stacey
Qi, Yiping
Pierick, Christopher J.
Hoffman, Ellen
Maeder, Morgan L.
Khayter, Cyd
Reyon, Deepak
Dobbs, Drena
Langenau, David M.
Stupar, Robert M.
Giraldez, Antonio J.
Voytas, Daniel F.
Peterson, Randall T.
Yeh, Jing-Ruey J.
Joung, J. Keith
Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title_full Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title_fullStr Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title_full_unstemmed Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title_short Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
title_sort selection-free zinc-finger nuclease engineering by context-dependent assembly (coda)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018472/
https://www.ncbi.nlm.nih.gov/pubmed/21151135
http://dx.doi.org/10.1038/nmeth.1542
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