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miRNeye: a microRNA expression atlas of the mouse eye
BACKGROUND: MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. RESULTS: In this report, we present the first comprehensive survey of miRNA expression in...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018480/ https://www.ncbi.nlm.nih.gov/pubmed/21171988 http://dx.doi.org/10.1186/1471-2164-11-715 |
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author | Karali, Marianthi Peluso, Ivana Gennarino, Vincenzo A Bilio, Marchesa Verde, Roberta Lago, Giampiero Dollé, Pascal Banfi, Sandro |
author_facet | Karali, Marianthi Peluso, Ivana Gennarino, Vincenzo A Bilio, Marchesa Verde, Roberta Lago, Giampiero Dollé, Pascal Banfi, Sandro |
author_sort | Karali, Marianthi |
collection | PubMed |
description | BACKGROUND: MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. RESULTS: In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. CONCLUSIONS: This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures. |
format | Text |
id | pubmed-3018480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30184802011-01-11 miRNeye: a microRNA expression atlas of the mouse eye Karali, Marianthi Peluso, Ivana Gennarino, Vincenzo A Bilio, Marchesa Verde, Roberta Lago, Giampiero Dollé, Pascal Banfi, Sandro BMC Genomics Research Article BACKGROUND: MicroRNAs (miRNAs) are key regulators of biological processes. To define miRNA function in the eye, it is essential to determine a high-resolution profile of their spatial and temporal distribution. RESULTS: In this report, we present the first comprehensive survey of miRNA expression in ocular tissues, using both microarray and RNA in situ hybridization (ISH) procedures. We initially determined the expression profiles of miRNAs in the retina, lens, cornea and retinal pigment epithelium of the adult mouse eye by microarray. Each tissue exhibited notably distinct miRNA enrichment patterns and cluster analysis identified groups of miRNAs that showed predominant expression in specific ocular tissues or combinations of them. Next, we performed RNA ISH for over 220 miRNAs, including those showing the highest expression levels by microarray, and generated a high-resolution expression atlas of miRNAs in the developing and adult wild-type mouse eye, which is accessible in the form of a publicly available web database. We found that 122 miRNAs displayed restricted expression domains in the eye at different developmental stages, with the majority of them expressed in one or more cell layers of the neural retina. CONCLUSIONS: This analysis revealed miRNAs with differential expression in ocular tissues and provided a detailed atlas of their tissue-specific distribution during development of the murine eye. The combination of the two approaches offers a valuable resource to decipher the contributions of specific miRNAs and miRNA clusters to the development of distinct ocular structures. BioMed Central 2010-12-20 /pmc/articles/PMC3018480/ /pubmed/21171988 http://dx.doi.org/10.1186/1471-2164-11-715 Text en Copyright ©2010 Karali et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Karali, Marianthi Peluso, Ivana Gennarino, Vincenzo A Bilio, Marchesa Verde, Roberta Lago, Giampiero Dollé, Pascal Banfi, Sandro miRNeye: a microRNA expression atlas of the mouse eye |
title | miRNeye: a microRNA expression atlas of the mouse eye |
title_full | miRNeye: a microRNA expression atlas of the mouse eye |
title_fullStr | miRNeye: a microRNA expression atlas of the mouse eye |
title_full_unstemmed | miRNeye: a microRNA expression atlas of the mouse eye |
title_short | miRNeye: a microRNA expression atlas of the mouse eye |
title_sort | mirneye: a microrna expression atlas of the mouse eye |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018480/ https://www.ncbi.nlm.nih.gov/pubmed/21171988 http://dx.doi.org/10.1186/1471-2164-11-715 |
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