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Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes we...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Humana Press Inc
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018597/ https://www.ncbi.nlm.nih.gov/pubmed/20526695 http://dx.doi.org/10.1007/s12031-010-9397-y |
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author | Alexandre, David Alonzeau, Jessy Bill, Brent R. Ekker, Stephen C. Waschek, James A. |
author_facet | Alexandre, David Alonzeau, Jessy Bill, Brent R. Ekker, Stephen C. Waschek, James A. |
author_sort | Alexandre, David |
collection | PubMed |
description | This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development. |
format | Text |
id | pubmed-3018597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Humana Press Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-30185972011-02-08 Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos Alexandre, David Alonzeau, Jessy Bill, Brent R. Ekker, Stephen C. Waschek, James A. J Mol Neurosci Article This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development. Humana Press Inc 2010-06-05 2011 /pmc/articles/PMC3018597/ /pubmed/20526695 http://dx.doi.org/10.1007/s12031-010-9397-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Alexandre, David Alonzeau, Jessy Bill, Brent R. Ekker, Stephen C. Waschek, James A. Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title | Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title_full | Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title_fullStr | Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title_full_unstemmed | Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title_short | Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos |
title_sort | expression analysis of pac1-r and pacap genes in zebrafish embryos |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018597/ https://www.ncbi.nlm.nih.gov/pubmed/20526695 http://dx.doi.org/10.1007/s12031-010-9397-y |
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