Cargando…

Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos

This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes we...

Descripción completa

Detalles Bibliográficos
Autores principales: Alexandre, David, Alonzeau, Jessy, Bill, Brent R., Ekker, Stephen C., Waschek, James A.
Formato: Texto
Lenguaje:English
Publicado: Humana Press Inc 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018597/
https://www.ncbi.nlm.nih.gov/pubmed/20526695
http://dx.doi.org/10.1007/s12031-010-9397-y
_version_ 1782196098909077504
author Alexandre, David
Alonzeau, Jessy
Bill, Brent R.
Ekker, Stephen C.
Waschek, James A.
author_facet Alexandre, David
Alonzeau, Jessy
Bill, Brent R.
Ekker, Stephen C.
Waschek, James A.
author_sort Alexandre, David
collection PubMed
description This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development.
format Text
id pubmed-3018597
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Humana Press Inc
record_format MEDLINE/PubMed
spelling pubmed-30185972011-02-08 Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos Alexandre, David Alonzeau, Jessy Bill, Brent R. Ekker, Stephen C. Waschek, James A. J Mol Neurosci Article This study describes the expression of the pituitary adenylate cyclase-activating polypeptide (PACAP1 and PACAP2) and PAC1 receptor genes (PAC1a-R and PAC1b-R) in the brain of zebrafish (Danio rerio) during development. In situ hybridization of the 24- and 48-hpf embryos revealed that PACAP genes were expressed in the telencephalon, the diencephalon, the rhombencephalon, and the neurons in the dorsal part of the spinal cord. PACAP2 mRNA appears to be the most abundant form during brain development. The two PAC1-R subtypes showed a similar expression pattern: mRNAs were detected in the forebrain, the thalamus, and the rhombencephalon. However, in the tectum, only PAC1b-R gene was detected. These results suggest that, in fish, PACAP may play a role in brain development. Humana Press Inc 2010-06-05 2011 /pmc/articles/PMC3018597/ /pubmed/20526695 http://dx.doi.org/10.1007/s12031-010-9397-y Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Alexandre, David
Alonzeau, Jessy
Bill, Brent R.
Ekker, Stephen C.
Waschek, James A.
Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title_full Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title_fullStr Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title_full_unstemmed Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title_short Expression Analysis of PAC1-R and PACAP Genes in Zebrafish Embryos
title_sort expression analysis of pac1-r and pacap genes in zebrafish embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018597/
https://www.ncbi.nlm.nih.gov/pubmed/20526695
http://dx.doi.org/10.1007/s12031-010-9397-y
work_keys_str_mv AT alexandredavid expressionanalysisofpac1randpacapgenesinzebrafishembryos
AT alonzeaujessy expressionanalysisofpac1randpacapgenesinzebrafishembryos
AT billbrentr expressionanalysisofpac1randpacapgenesinzebrafishembryos
AT ekkerstephenc expressionanalysisofpac1randpacapgenesinzebrafishembryos
AT waschekjamesa expressionanalysisofpac1randpacapgenesinzebrafishembryos