An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

BACKGROUND: The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening. Purification of recombinant protein in sufficient yield and purity is a tedious undertaking and can be demanding especially...

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Detalles Bibliográficos
Autores principales: Dreyer, Anita M, Beauchamp, Jeremy, Matile, Hugues, Pluschke, Gerd
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019159/
https://www.ncbi.nlm.nih.gov/pubmed/21159168
http://dx.doi.org/10.1186/1472-6750-10-87

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019159/
https://www.ncbi.nlm.nih.gov/pubmed/21159168
http://dx.doi.org/10.1186/1472-6750-10-87

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