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Changes in the mucosal barrier during acute and chronic Trichuris muris infection

The intestinal mucosal barrier, part of the innate immune defence, is responsive to the external environment and changes in response to infection. There is disparate evidence for the epithelial and goblet cell products within the intrinsic barrier being part of a response to resolve infection. We co...

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Autores principales: Hasnain, S Z, Thornton, D J, Grencis, R K
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020324/
https://www.ncbi.nlm.nih.gov/pubmed/21155842
http://dx.doi.org/10.1111/j.1365-3024.2010.01258.x
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author Hasnain, S Z
Thornton, D J
Grencis, R K
author_facet Hasnain, S Z
Thornton, D J
Grencis, R K
author_sort Hasnain, S Z
collection PubMed
description The intestinal mucosal barrier, part of the innate immune defence, is responsive to the external environment and changes in response to infection. There is disparate evidence for the epithelial and goblet cell products within the intrinsic barrier being part of a response to resolve infection. We comprehensively analysed the changes of mucosal glycoconjugates during acute and chronic infection by utilising the Trichuris muris (T. muris) model. Transcription factors, atonal homolog 1 (Math-1) and SAM pointed domain containing ETS transcription factor (Spdef) were activated during acute infection, which promoted stem cell fate towards a secretory cell phenotype. The thickness of the intermediate barrier, the carbohydrate-rich glycocalyx, composed of cell surface mucins increased with exposure to T. muris, with an increase in Muc4, Muc13 and Muc17. Overall, hypersecretion of glycoproteins into the extrinsic barrier (mediated by IL-13) via the gamma amino-butyric acid-α3 receptor (GABA-α3), was observed during acute infection. Furthermore, altered glycosylation was observed during acute and chronic infection; mucins were more highly charged during acute infection than during chronic infection. This study readdresses the changes within the mucosal barrier, in particular in the cell surface and secreted mucins during acute and chronic nematode infection.
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spelling pubmed-30203242011-01-19 Changes in the mucosal barrier during acute and chronic Trichuris muris infection Hasnain, S Z Thornton, D J Grencis, R K Parasite Immunol Original Articles The intestinal mucosal barrier, part of the innate immune defence, is responsive to the external environment and changes in response to infection. There is disparate evidence for the epithelial and goblet cell products within the intrinsic barrier being part of a response to resolve infection. We comprehensively analysed the changes of mucosal glycoconjugates during acute and chronic infection by utilising the Trichuris muris (T. muris) model. Transcription factors, atonal homolog 1 (Math-1) and SAM pointed domain containing ETS transcription factor (Spdef) were activated during acute infection, which promoted stem cell fate towards a secretory cell phenotype. The thickness of the intermediate barrier, the carbohydrate-rich glycocalyx, composed of cell surface mucins increased with exposure to T. muris, with an increase in Muc4, Muc13 and Muc17. Overall, hypersecretion of glycoproteins into the extrinsic barrier (mediated by IL-13) via the gamma amino-butyric acid-α3 receptor (GABA-α3), was observed during acute infection. Furthermore, altered glycosylation was observed during acute and chronic infection; mucins were more highly charged during acute infection than during chronic infection. This study readdresses the changes within the mucosal barrier, in particular in the cell surface and secreted mucins during acute and chronic nematode infection. Blackwell Publishing Ltd 2011-01 /pmc/articles/PMC3020324/ /pubmed/21155842 http://dx.doi.org/10.1111/j.1365-3024.2010.01258.x Text en Copyright © 2011 Blackwell Publishing Ltd http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Original Articles
Hasnain, S Z
Thornton, D J
Grencis, R K
Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title_full Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title_fullStr Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title_full_unstemmed Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title_short Changes in the mucosal barrier during acute and chronic Trichuris muris infection
title_sort changes in the mucosal barrier during acute and chronic trichuris muris infection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020324/
https://www.ncbi.nlm.nih.gov/pubmed/21155842
http://dx.doi.org/10.1111/j.1365-3024.2010.01258.x
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