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Salivary Gland NK Cells Are Phenotypically and Functionally Unique
Natural killer (NK) cells and CD8(+) T cells play vital roles in containing and eliminating systemic cytomegalovirus (CMV). However, CMV has a tropism for the salivary gland acinar epithelial cells and persists in this organ for several weeks after primary infection. Here we characterize a distinct...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020929/ https://www.ncbi.nlm.nih.gov/pubmed/21249177 http://dx.doi.org/10.1371/journal.ppat.1001254 |
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author | Tessmer, Marlowe S. Reilly, Emma C. Brossay, Laurent |
author_facet | Tessmer, Marlowe S. Reilly, Emma C. Brossay, Laurent |
author_sort | Tessmer, Marlowe S. |
collection | PubMed |
description | Natural killer (NK) cells and CD8(+) T cells play vital roles in containing and eliminating systemic cytomegalovirus (CMV). However, CMV has a tropism for the salivary gland acinar epithelial cells and persists in this organ for several weeks after primary infection. Here we characterize a distinct NK cell population that resides in the salivary gland, uncommon to any described to date, expressing both mature and immature NK cell markers. Using RORγt reporter mice and nude mice, we also show that the salivary gland NK cells are not lymphoid tissue inducer NK-like cells and are not thymic derived. During the course of murine cytomegalovirus (MCMV) infection, we found that salivary gland NK cells detect the infection and acquire activation markers, but have limited capacity to produce IFN-γ and degranulate. Salivary gland NK cell effector functions are not regulated by iNKT or T(reg) cells, which are mostly absent in the salivary gland. Additionally, we demonstrate that peripheral NK cells are not recruited to this organ even after the systemic infection has been controlled. Altogether, these results indicate that viral persistence and latency in the salivary glands may be due in part to the presence of unfit NK cells and the lack of recruitment of peripheral NK cells. |
format | Text |
id | pubmed-3020929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30209292011-01-19 Salivary Gland NK Cells Are Phenotypically and Functionally Unique Tessmer, Marlowe S. Reilly, Emma C. Brossay, Laurent PLoS Pathog Research Article Natural killer (NK) cells and CD8(+) T cells play vital roles in containing and eliminating systemic cytomegalovirus (CMV). However, CMV has a tropism for the salivary gland acinar epithelial cells and persists in this organ for several weeks after primary infection. Here we characterize a distinct NK cell population that resides in the salivary gland, uncommon to any described to date, expressing both mature and immature NK cell markers. Using RORγt reporter mice and nude mice, we also show that the salivary gland NK cells are not lymphoid tissue inducer NK-like cells and are not thymic derived. During the course of murine cytomegalovirus (MCMV) infection, we found that salivary gland NK cells detect the infection and acquire activation markers, but have limited capacity to produce IFN-γ and degranulate. Salivary gland NK cell effector functions are not regulated by iNKT or T(reg) cells, which are mostly absent in the salivary gland. Additionally, we demonstrate that peripheral NK cells are not recruited to this organ even after the systemic infection has been controlled. Altogether, these results indicate that viral persistence and latency in the salivary glands may be due in part to the presence of unfit NK cells and the lack of recruitment of peripheral NK cells. Public Library of Science 2011-01-13 /pmc/articles/PMC3020929/ /pubmed/21249177 http://dx.doi.org/10.1371/journal.ppat.1001254 Text en Tessmer et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tessmer, Marlowe S. Reilly, Emma C. Brossay, Laurent Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title | Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title_full | Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title_fullStr | Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title_full_unstemmed | Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title_short | Salivary Gland NK Cells Are Phenotypically and Functionally Unique |
title_sort | salivary gland nk cells are phenotypically and functionally unique |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020929/ https://www.ncbi.nlm.nih.gov/pubmed/21249177 http://dx.doi.org/10.1371/journal.ppat.1001254 |
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