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Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1

The transient receptor potential vanilloid type 1 (TRPV1) channel is a well recognized polymodal signal detector that is activated by painful stimuli such as capsaicin. Here, we show that TRPV1 is expressed in the lateral nucleus of the amygdala (LA). Despite the fact that the central amygdala displ...

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Autores principales: Zschenderlein, Carsten, Gebhardt, Christine, von Bohlen und Halbach, Oliver, Kulisch, Christoph, Albrecht, Doris
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020947/
https://www.ncbi.nlm.nih.gov/pubmed/21249195
http://dx.doi.org/10.1371/journal.pone.0016116
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author Zschenderlein, Carsten
Gebhardt, Christine
von Bohlen und Halbach, Oliver
Kulisch, Christoph
Albrecht, Doris
author_facet Zschenderlein, Carsten
Gebhardt, Christine
von Bohlen und Halbach, Oliver
Kulisch, Christoph
Albrecht, Doris
author_sort Zschenderlein, Carsten
collection PubMed
description The transient receptor potential vanilloid type 1 (TRPV1) channel is a well recognized polymodal signal detector that is activated by painful stimuli such as capsaicin. Here, we show that TRPV1 is expressed in the lateral nucleus of the amygdala (LA). Despite the fact that the central amygdala displays the highest neuronal density, the highest density of TRPV1 labeled neurons was found within the nuclei of the basolateral complex of the amygdala. Capsaicin specifically changed the magnitude of long-term potentiation (LTP) in the LA in brain slices of mice depending on the anesthetic (ether, isoflurane) used before euthanasia. After ether anesthesia, capsaicin had a suppressive effect on LA-LTP both in patch clamp and in extracellular recordings. The capsaicin-induced reduction of LTP was completely blocked by the nitric oxide synthase (NOS) inhibitor L-NAME and was absent in neuronal NOS as well as in TRPV1 deficient mice. The specific antagonist of cannabinoid receptor type 1 (CB1), AM 251, was also able to reduce the inhibitory effect of capsaicin on LA-LTP, suggesting that stimulation of TRPV1 provokes the generation of anandamide in the brain which seems to inhibit NO synthesis. After isoflurane anesthesia before euthanasia capsaicin caused a TRPV1-mediated increase in the magnitude of LA-LTP. Therefore, our results also indicate that the appropriate choice of the anesthetics used is an important consideration when brain plasticity and the action of endovanilloids will be evaluated. In summary, our results demonstrate that TRPV1 may be involved in the amygdala control of learning mechanisms.
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spelling pubmed-30209472011-01-19 Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1 Zschenderlein, Carsten Gebhardt, Christine von Bohlen und Halbach, Oliver Kulisch, Christoph Albrecht, Doris PLoS One Research Article The transient receptor potential vanilloid type 1 (TRPV1) channel is a well recognized polymodal signal detector that is activated by painful stimuli such as capsaicin. Here, we show that TRPV1 is expressed in the lateral nucleus of the amygdala (LA). Despite the fact that the central amygdala displays the highest neuronal density, the highest density of TRPV1 labeled neurons was found within the nuclei of the basolateral complex of the amygdala. Capsaicin specifically changed the magnitude of long-term potentiation (LTP) in the LA in brain slices of mice depending on the anesthetic (ether, isoflurane) used before euthanasia. After ether anesthesia, capsaicin had a suppressive effect on LA-LTP both in patch clamp and in extracellular recordings. The capsaicin-induced reduction of LTP was completely blocked by the nitric oxide synthase (NOS) inhibitor L-NAME and was absent in neuronal NOS as well as in TRPV1 deficient mice. The specific antagonist of cannabinoid receptor type 1 (CB1), AM 251, was also able to reduce the inhibitory effect of capsaicin on LA-LTP, suggesting that stimulation of TRPV1 provokes the generation of anandamide in the brain which seems to inhibit NO synthesis. After isoflurane anesthesia before euthanasia capsaicin caused a TRPV1-mediated increase in the magnitude of LA-LTP. Therefore, our results also indicate that the appropriate choice of the anesthetics used is an important consideration when brain plasticity and the action of endovanilloids will be evaluated. In summary, our results demonstrate that TRPV1 may be involved in the amygdala control of learning mechanisms. Public Library of Science 2011-01-13 /pmc/articles/PMC3020947/ /pubmed/21249195 http://dx.doi.org/10.1371/journal.pone.0016116 Text en Zschenderlein et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zschenderlein, Carsten
Gebhardt, Christine
von Bohlen und Halbach, Oliver
Kulisch, Christoph
Albrecht, Doris
Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title_full Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title_fullStr Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title_full_unstemmed Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title_short Capsaicin-Induced Changes in LTP in the Lateral Amygdala Are Mediated by TRPV1
title_sort capsaicin-induced changes in ltp in the lateral amygdala are mediated by trpv1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020947/
https://www.ncbi.nlm.nih.gov/pubmed/21249195
http://dx.doi.org/10.1371/journal.pone.0016116
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