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Generation of Human Melanocytes from Induced Pluripotent Stem Cells
Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiatio...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020956/ https://www.ncbi.nlm.nih.gov/pubmed/21249204 http://dx.doi.org/10.1371/journal.pone.0016182 |
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author | Ohta, Shigeki Imaizumi, Yoichi Okada, Yohei Akamatsu, Wado Kuwahara, Reiko Ohyama, Manabu Amagai, Masayuki Matsuzaki, Yumi Yamanaka, Shinya Okano, Hideyuki Kawakami, Yutaka |
author_facet | Ohta, Shigeki Imaizumi, Yoichi Okada, Yohei Akamatsu, Wado Kuwahara, Reiko Ohyama, Manabu Amagai, Masayuki Matsuzaki, Yumi Yamanaka, Shinya Okano, Hideyuki Kawakami, Yutaka |
author_sort | Ohta, Shigeki |
collection | PubMed |
description | Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation and the defining characteristics of melanocyte stem cells in humans are, however, largely unknown. In the present study, we set out to generate melanocytes from human iPS cells in vitro, leading to a preliminary investigation of the mechanisms of human melanocyte differentiation. We generated iPS cell lines from human dermal fibroblasts using the Yamanaka factors (SOX2, OCT3/4, and KLF4, with or without c-MYC). These iPS cell lines were subsequently used to form embryoid bodies (EBs) and then differentiated into melanocytes via culture supplementation with Wnt3a, SCF, and ET-3. Seven weeks after inducing differentiation, pigmented cells expressing melanocyte markers such as MITF, tyrosinase, SILV, and TYRP1, were detected. Melanosomes were identified in these pigmented cells by electron microscopy, and global gene expression profiling of the pigmented cells showed a high similarity to that of human primary foreskin-derived melanocytes, suggesting the successful generation of melanocytes from iPS cells. This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma. |
format | Text |
id | pubmed-3020956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30209562011-01-19 Generation of Human Melanocytes from Induced Pluripotent Stem Cells Ohta, Shigeki Imaizumi, Yoichi Okada, Yohei Akamatsu, Wado Kuwahara, Reiko Ohyama, Manabu Amagai, Masayuki Matsuzaki, Yumi Yamanaka, Shinya Okano, Hideyuki Kawakami, Yutaka PLoS One Research Article Epidermal melanocytes play an important role in protecting the skin from UV rays, and their functional impairment results in pigment disorders. Additionally, melanomas are considered to arise from mutations that accumulate in melanocyte stem cells. The mechanisms underlying melanocyte differentiation and the defining characteristics of melanocyte stem cells in humans are, however, largely unknown. In the present study, we set out to generate melanocytes from human iPS cells in vitro, leading to a preliminary investigation of the mechanisms of human melanocyte differentiation. We generated iPS cell lines from human dermal fibroblasts using the Yamanaka factors (SOX2, OCT3/4, and KLF4, with or without c-MYC). These iPS cell lines were subsequently used to form embryoid bodies (EBs) and then differentiated into melanocytes via culture supplementation with Wnt3a, SCF, and ET-3. Seven weeks after inducing differentiation, pigmented cells expressing melanocyte markers such as MITF, tyrosinase, SILV, and TYRP1, were detected. Melanosomes were identified in these pigmented cells by electron microscopy, and global gene expression profiling of the pigmented cells showed a high similarity to that of human primary foreskin-derived melanocytes, suggesting the successful generation of melanocytes from iPS cells. This in vitro differentiation system should prove useful for understanding human melanocyte biology and revealing the mechanism of various pigment cell disorders, including melanoma. Public Library of Science 2011-01-13 /pmc/articles/PMC3020956/ /pubmed/21249204 http://dx.doi.org/10.1371/journal.pone.0016182 Text en Ohta et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ohta, Shigeki Imaizumi, Yoichi Okada, Yohei Akamatsu, Wado Kuwahara, Reiko Ohyama, Manabu Amagai, Masayuki Matsuzaki, Yumi Yamanaka, Shinya Okano, Hideyuki Kawakami, Yutaka Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title | Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title_full | Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title_fullStr | Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title_full_unstemmed | Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title_short | Generation of Human Melanocytes from Induced Pluripotent Stem Cells |
title_sort | generation of human melanocytes from induced pluripotent stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3020956/ https://www.ncbi.nlm.nih.gov/pubmed/21249204 http://dx.doi.org/10.1371/journal.pone.0016182 |
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