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Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential

Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long b...

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Autores principales: de Souza Faloni, Ana Paula, Schoenmaker, Ton, Azari, Azin, Katchburian, Eduardo, Cerri, Paulo S., de Vries, Teun J., Everts, Vincent
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021190/
https://www.ncbi.nlm.nih.gov/pubmed/20862464
http://dx.doi.org/10.1007/s00223-010-9418-4
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author de Souza Faloni, Ana Paula
Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo
Cerri, Paulo S.
de Vries, Teun J.
Everts, Vincent
author_facet de Souza Faloni, Ana Paula
Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo
Cerri, Paulo S.
de Vries, Teun J.
Everts, Vincent
author_sort de Souza Faloni, Ana Paula
collection PubMed
description Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. The expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. The jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. In conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.
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spelling pubmed-30211902011-02-22 Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential de Souza Faloni, Ana Paula Schoenmaker, Ton Azari, Azin Katchburian, Eduardo Cerri, Paulo S. de Vries, Teun J. Everts, Vincent Calcif Tissue Int Article Osteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. The expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. The jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. In conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow. Springer-Verlag 2010-09-23 2011 /pmc/articles/PMC3021190/ /pubmed/20862464 http://dx.doi.org/10.1007/s00223-010-9418-4 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
de Souza Faloni, Ana Paula
Schoenmaker, Ton
Azari, Azin
Katchburian, Eduardo
Cerri, Paulo S.
de Vries, Teun J.
Everts, Vincent
Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_full Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_fullStr Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_full_unstemmed Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_short Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
title_sort jaw and long bone marrows have a different osteoclastogenic potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021190/
https://www.ncbi.nlm.nih.gov/pubmed/20862464
http://dx.doi.org/10.1007/s00223-010-9418-4
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