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Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome
Direct manipulation of bacterial chromosomes by recombination-based techniques has become increasingly important for both cognitive and applied research. Here we demonstrate, for the first time, the combination of the Red recombinase system with I-SceI endonuclease-based selection of successful reco...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021506/ https://www.ncbi.nlm.nih.gov/pubmed/21264289 http://dx.doi.org/10.1371/journal.pone.0015763 |