Cargando…

FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation

BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using flu...

Descripción completa

Detalles Bibliográficos
Autores principales: Kosuge, Hisanori, Sherlock, Sarah P., Kitagawa, Toshiro, Terashima, Masahiro, Barral, Joëlle K., Nishimura, Dwight G., Dai, Hongjie, McConnell, Michael V.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021517/
https://www.ncbi.nlm.nih.gov/pubmed/21264237
http://dx.doi.org/10.1371/journal.pone.0014523
_version_ 1782196386613166080
author Kosuge, Hisanori
Sherlock, Sarah P.
Kitagawa, Toshiro
Terashima, Masahiro
Barral, Joëlle K.
Nishimura, Dwight G.
Dai, Hongjie
McConnell, Michael V.
author_facet Kosuge, Hisanori
Sherlock, Sarah P.
Kitagawa, Toshiro
Terashima, Masahiro
Barral, Joëlle K.
Nishimura, Dwight G.
Dai, Hongjie
McConnell, Michael V.
author_sort Kosuge, Hisanori
collection PubMed
description BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using fluorescence and MRI. METHODS AND RESULTS: Hyperlipidemic and diabetic mice underwent carotid ligation to produce a macrophage-rich vascular lesion. In situ and ex vivo fluorescence imaging were performed at 48 hours after intravenous injection of FeCo/GC conjugated to Cy5.5 (n = 8, 8 nmol of Cy5.5/mouse). Significant fluorescence signal from FeCo/GC-Cy5.5 was present in the ligated left carotid arteries, but not in the control (non-ligated) right carotid arteries or sham-operated carotid arteries (p = 0.03 for ligated vs. non-ligated). Serial in vivo 3T MRI was performed at 48 and 72 hours after intravenous FeCo/GC (n = 6, 270 µg Fe/mouse). Significant T2* signal loss from FeCo/GC was seen in ligated left carotid arteries, not in non-ligated controls (p = 0.03). Immunofluorescence staining showed colocalization of FeCo/GC and macrophages in ligated carotid arteries. CONCLUSIONS: FeCo/GC accumulates in vascular macrophages in vivo, allowing fluorescence and MR imaging. This multi-functional high-relaxivity nanoparticle platform provides a promising approach for cellular imaging of vascular inflammation.
format Text
id pubmed-3021517
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30215172011-01-24 FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation Kosuge, Hisanori Sherlock, Sarah P. Kitagawa, Toshiro Terashima, Masahiro Barral, Joëlle K. Nishimura, Dwight G. Dai, Hongjie McConnell, Michael V. PLoS One Research Article BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using fluorescence and MRI. METHODS AND RESULTS: Hyperlipidemic and diabetic mice underwent carotid ligation to produce a macrophage-rich vascular lesion. In situ and ex vivo fluorescence imaging were performed at 48 hours after intravenous injection of FeCo/GC conjugated to Cy5.5 (n = 8, 8 nmol of Cy5.5/mouse). Significant fluorescence signal from FeCo/GC-Cy5.5 was present in the ligated left carotid arteries, but not in the control (non-ligated) right carotid arteries or sham-operated carotid arteries (p = 0.03 for ligated vs. non-ligated). Serial in vivo 3T MRI was performed at 48 and 72 hours after intravenous FeCo/GC (n = 6, 270 µg Fe/mouse). Significant T2* signal loss from FeCo/GC was seen in ligated left carotid arteries, not in non-ligated controls (p = 0.03). Immunofluorescence staining showed colocalization of FeCo/GC and macrophages in ligated carotid arteries. CONCLUSIONS: FeCo/GC accumulates in vascular macrophages in vivo, allowing fluorescence and MR imaging. This multi-functional high-relaxivity nanoparticle platform provides a promising approach for cellular imaging of vascular inflammation. Public Library of Science 2011-01-14 /pmc/articles/PMC3021517/ /pubmed/21264237 http://dx.doi.org/10.1371/journal.pone.0014523 Text en Kosuge et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kosuge, Hisanori
Sherlock, Sarah P.
Kitagawa, Toshiro
Terashima, Masahiro
Barral, Joëlle K.
Nishimura, Dwight G.
Dai, Hongjie
McConnell, Michael V.
FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title_full FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title_fullStr FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title_full_unstemmed FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title_short FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
title_sort feco/graphite nanocrystals for multi-modality imaging of experimental vascular inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021517/
https://www.ncbi.nlm.nih.gov/pubmed/21264237
http://dx.doi.org/10.1371/journal.pone.0014523
work_keys_str_mv AT kosugehisanori fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT sherlocksarahp fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT kitagawatoshiro fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT terashimamasahiro fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT barraljoellek fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT nishimuradwightg fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT daihongjie fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation
AT mcconnellmichaelv fecographitenanocrystalsformultimodalityimagingofexperimentalvascularinflammation