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FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation
BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using flu...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021517/ https://www.ncbi.nlm.nih.gov/pubmed/21264237 http://dx.doi.org/10.1371/journal.pone.0014523 |
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author | Kosuge, Hisanori Sherlock, Sarah P. Kitagawa, Toshiro Terashima, Masahiro Barral, Joëlle K. Nishimura, Dwight G. Dai, Hongjie McConnell, Michael V. |
author_facet | Kosuge, Hisanori Sherlock, Sarah P. Kitagawa, Toshiro Terashima, Masahiro Barral, Joëlle K. Nishimura, Dwight G. Dai, Hongjie McConnell, Michael V. |
author_sort | Kosuge, Hisanori |
collection | PubMed |
description | BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using fluorescence and MRI. METHODS AND RESULTS: Hyperlipidemic and diabetic mice underwent carotid ligation to produce a macrophage-rich vascular lesion. In situ and ex vivo fluorescence imaging were performed at 48 hours after intravenous injection of FeCo/GC conjugated to Cy5.5 (n = 8, 8 nmol of Cy5.5/mouse). Significant fluorescence signal from FeCo/GC-Cy5.5 was present in the ligated left carotid arteries, but not in the control (non-ligated) right carotid arteries or sham-operated carotid arteries (p = 0.03 for ligated vs. non-ligated). Serial in vivo 3T MRI was performed at 48 and 72 hours after intravenous FeCo/GC (n = 6, 270 µg Fe/mouse). Significant T2* signal loss from FeCo/GC was seen in ligated left carotid arteries, not in non-ligated controls (p = 0.03). Immunofluorescence staining showed colocalization of FeCo/GC and macrophages in ligated carotid arteries. CONCLUSIONS: FeCo/GC accumulates in vascular macrophages in vivo, allowing fluorescence and MR imaging. This multi-functional high-relaxivity nanoparticle platform provides a promising approach for cellular imaging of vascular inflammation. |
format | Text |
id | pubmed-3021517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30215172011-01-24 FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation Kosuge, Hisanori Sherlock, Sarah P. Kitagawa, Toshiro Terashima, Masahiro Barral, Joëlle K. Nishimura, Dwight G. Dai, Hongjie McConnell, Michael V. PLoS One Research Article BACKGROUND: FeCo/graphitic-carbon nanocrystals (FeCo/GC) are biocompatible, high-relaxivity, multi-functional nanoparticles. Macrophages represent important cellular imaging targets for assessing vascular inflammation. We evaluated FeCo/GC for vascular macrophage uptake and imaging in vivo using fluorescence and MRI. METHODS AND RESULTS: Hyperlipidemic and diabetic mice underwent carotid ligation to produce a macrophage-rich vascular lesion. In situ and ex vivo fluorescence imaging were performed at 48 hours after intravenous injection of FeCo/GC conjugated to Cy5.5 (n = 8, 8 nmol of Cy5.5/mouse). Significant fluorescence signal from FeCo/GC-Cy5.5 was present in the ligated left carotid arteries, but not in the control (non-ligated) right carotid arteries or sham-operated carotid arteries (p = 0.03 for ligated vs. non-ligated). Serial in vivo 3T MRI was performed at 48 and 72 hours after intravenous FeCo/GC (n = 6, 270 µg Fe/mouse). Significant T2* signal loss from FeCo/GC was seen in ligated left carotid arteries, not in non-ligated controls (p = 0.03). Immunofluorescence staining showed colocalization of FeCo/GC and macrophages in ligated carotid arteries. CONCLUSIONS: FeCo/GC accumulates in vascular macrophages in vivo, allowing fluorescence and MR imaging. This multi-functional high-relaxivity nanoparticle platform provides a promising approach for cellular imaging of vascular inflammation. Public Library of Science 2011-01-14 /pmc/articles/PMC3021517/ /pubmed/21264237 http://dx.doi.org/10.1371/journal.pone.0014523 Text en Kosuge et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kosuge, Hisanori Sherlock, Sarah P. Kitagawa, Toshiro Terashima, Masahiro Barral, Joëlle K. Nishimura, Dwight G. Dai, Hongjie McConnell, Michael V. FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title | FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title_full | FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title_fullStr | FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title_full_unstemmed | FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title_short | FeCo/Graphite Nanocrystals for Multi-Modality Imaging of Experimental Vascular Inflammation |
title_sort | feco/graphite nanocrystals for multi-modality imaging of experimental vascular inflammation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021517/ https://www.ncbi.nlm.nih.gov/pubmed/21264237 http://dx.doi.org/10.1371/journal.pone.0014523 |
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