Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules

Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric dat...

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Detalles Bibliográficos
Autores principales: Giang, Do T, Hoang, Vu D
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Pharmaceutical Analysis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021696/
https://www.ncbi.nlm.nih.gov/pubmed/21264124
http://dx.doi.org/10.4103/0975-1483.63168
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author Giang, Do T
Hoang, Vu D
author_facet Giang, Do T
Hoang, Vu D
author_sort Giang, Do T
collection PubMed
description Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis.
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spelling pubmed-30216962011-01-24 Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules Giang, Do T Hoang, Vu D J Young Pharm Pharmaceutical Analysis Reversed-phase HPLC and UV spectrophotometric techniques using water as solvent have been developed and validated for the simultaneous determination of amoxicillin and cloxacillin in capsules. For both techniques, the linearity range of 60.073x2013;140.0 µg/mL was studied. The spectrophotometric data show that non-derivative techniques, such as absorbance ratio and compensation, and ratio spectra first-order derivative could be successfully used for the co-assay of amoxicillin and cloxacillin. Based on the statistical comparison of spectrophotometric and chromatographic data, the interchangeability between HPLC and UV spectrophotometric techniques has been suggested for the routine analysis. Medknow Publications 2010 /pmc/articles/PMC3021696/ /pubmed/21264124 http://dx.doi.org/10.4103/0975-1483.63168 Text en © Journal of Young Pharmacists http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Pharmaceutical Analysis
Giang, Do T
Hoang, Vu D
Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title_full Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title_fullStr Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title_full_unstemmed Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title_short Comparative Study of RP-HPLC and UV Spectrophotometric Techniques for the Simultaneous Determination of Amoxicillin and Cloxacillin in Capsules
title_sort comparative study of rp-hplc and uv spectrophotometric techniques for the simultaneous determination of amoxicillin and cloxacillin in capsules
topic Pharmaceutical Analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021696/
https://www.ncbi.nlm.nih.gov/pubmed/21264124
http://dx.doi.org/10.4103/0975-1483.63168
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