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Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization

High levels of urokinase receptor (uPAR) in tissue and serum of patients with chondrosarcoma correlate with poor prognosis. First, we analyzed the uPAR levels in tissues and plasma of five patients affected by chondrosarcoma. Interestingly, very high levels of uPAR and its soluble forms (SuPAR) were...

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Autores principales: Bifulco, Katia, Longanesi-Cattani, Immacolata, Masucci, Maria Teresa, De Chiara, Annarosaria, Fazioli, Flavio, Di Carluccio, Gioconda, Pirozzi, Giuseppe, Gallo, Michele, La Rocca, Antonello, Apice, Gaetano, Rocco, Gaetano, Carriero, Maria Vincenza
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021884/
https://www.ncbi.nlm.nih.gov/pubmed/21253510
http://dx.doi.org/10.1155/2011/842842
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author Bifulco, Katia
Longanesi-Cattani, Immacolata
Masucci, Maria Teresa
De Chiara, Annarosaria
Fazioli, Flavio
Di Carluccio, Gioconda
Pirozzi, Giuseppe
Gallo, Michele
La Rocca, Antonello
Apice, Gaetano
Rocco, Gaetano
Carriero, Maria Vincenza
author_facet Bifulco, Katia
Longanesi-Cattani, Immacolata
Masucci, Maria Teresa
De Chiara, Annarosaria
Fazioli, Flavio
Di Carluccio, Gioconda
Pirozzi, Giuseppe
Gallo, Michele
La Rocca, Antonello
Apice, Gaetano
Rocco, Gaetano
Carriero, Maria Vincenza
author_sort Bifulco, Katia
collection PubMed
description High levels of urokinase receptor (uPAR) in tissue and serum of patients with chondrosarcoma correlate with poor prognosis. First, we analyzed the uPAR levels in tissues and plasma of five patients affected by chondrosarcoma. Interestingly, very high levels of uPAR and its soluble forms (SuPAR) were found on tumor cell surfaces and plasma, respectively, of two patients with lung metastases. Therefore, to investigate the role of SuPAR in chondrosaromas, we generated a primary cell culture from a chondrosarcoma tissue overexpressing uPAR on cell surfaces. We found that chondrosarcoma-like primary culture cells release a large amount of SuPAR in the medium. In vitro, SuPAR elicits chondrosarcoma cell migration likely through its uPAR(88-92) sequence, since the DII(88-183) or DIIDIIR(88-284) uPAR domains retain motogen effect whereas DI(1-87) or DIII(184-284) domains, both lacking the uPAR(88-92) sequence, are ineffective. Chondrosarcoma cells cross matrigel in response to SuPAR, and their invasion capability is abrogated by RERF peptide which inhibits uPAR(88-92) signalling. These findings assign a role to uPAR in mobilizing chondrosarcoma cells and suggest that RERF peptide may be regarded as a prototype to generate new therapeutics for the chondrosarcoma treatment.
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spelling pubmed-30218842011-01-20 Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization Bifulco, Katia Longanesi-Cattani, Immacolata Masucci, Maria Teresa De Chiara, Annarosaria Fazioli, Flavio Di Carluccio, Gioconda Pirozzi, Giuseppe Gallo, Michele La Rocca, Antonello Apice, Gaetano Rocco, Gaetano Carriero, Maria Vincenza Sarcoma Research Article High levels of urokinase receptor (uPAR) in tissue and serum of patients with chondrosarcoma correlate with poor prognosis. First, we analyzed the uPAR levels in tissues and plasma of five patients affected by chondrosarcoma. Interestingly, very high levels of uPAR and its soluble forms (SuPAR) were found on tumor cell surfaces and plasma, respectively, of two patients with lung metastases. Therefore, to investigate the role of SuPAR in chondrosaromas, we generated a primary cell culture from a chondrosarcoma tissue overexpressing uPAR on cell surfaces. We found that chondrosarcoma-like primary culture cells release a large amount of SuPAR in the medium. In vitro, SuPAR elicits chondrosarcoma cell migration likely through its uPAR(88-92) sequence, since the DII(88-183) or DIIDIIR(88-284) uPAR domains retain motogen effect whereas DI(1-87) or DIII(184-284) domains, both lacking the uPAR(88-92) sequence, are ineffective. Chondrosarcoma cells cross matrigel in response to SuPAR, and their invasion capability is abrogated by RERF peptide which inhibits uPAR(88-92) signalling. These findings assign a role to uPAR in mobilizing chondrosarcoma cells and suggest that RERF peptide may be regarded as a prototype to generate new therapeutics for the chondrosarcoma treatment. Hindawi Publishing Corporation 2011 2010-12-30 /pmc/articles/PMC3021884/ /pubmed/21253510 http://dx.doi.org/10.1155/2011/842842 Text en Copyright © 2011 Katia Bifulco et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bifulco, Katia
Longanesi-Cattani, Immacolata
Masucci, Maria Teresa
De Chiara, Annarosaria
Fazioli, Flavio
Di Carluccio, Gioconda
Pirozzi, Giuseppe
Gallo, Michele
La Rocca, Antonello
Apice, Gaetano
Rocco, Gaetano
Carriero, Maria Vincenza
Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title_full Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title_fullStr Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title_full_unstemmed Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title_short Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization
title_sort involvement of the soluble urokinase receptor in chondrosarcoma cell mobilization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021884/
https://www.ncbi.nlm.nih.gov/pubmed/21253510
http://dx.doi.org/10.1155/2011/842842
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