Cargando…

Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils

Aims. To establish a new model of zymosan-induced temporomandibular joint (TMJ) arthritis in the rat and to investigate the role of nitric oxide. Methods. Inflammation was induced by an intra-articular injection of zymosan into the left TMJ. Mechanical hypernociception, cell influx, vascular permeab...

Descripción completa

Detalles Bibliográficos
Autores principales: Chaves, Hellíada Vasconcelos, Ribeiro, Ronaldo de Albuquerque, de Souza, André Mattos Brito, Silva, Antonio Alfredo Rodrigues e, Gomes, Antoniella Souza, Vale, Mariana Lima, Bezerra, Mirna Marques, Brito, Gerly Anne de Castro
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022228/
https://www.ncbi.nlm.nih.gov/pubmed/21274271
http://dx.doi.org/10.1155/2011/707985
_version_ 1782196488713011200
author Chaves, Hellíada Vasconcelos
Ribeiro, Ronaldo de Albuquerque
de Souza, André Mattos Brito
Silva, Antonio Alfredo Rodrigues e
Gomes, Antoniella Souza
Vale, Mariana Lima
Bezerra, Mirna Marques
Brito, Gerly Anne de Castro
author_facet Chaves, Hellíada Vasconcelos
Ribeiro, Ronaldo de Albuquerque
de Souza, André Mattos Brito
Silva, Antonio Alfredo Rodrigues e
Gomes, Antoniella Souza
Vale, Mariana Lima
Bezerra, Mirna Marques
Brito, Gerly Anne de Castro
author_sort Chaves, Hellíada Vasconcelos
collection PubMed
description Aims. To establish a new model of zymosan-induced temporomandibular joint (TMJ) arthritis in the rat and to investigate the role of nitric oxide. Methods. Inflammation was induced by an intra-articular injection of zymosan into the left TMJ. Mechanical hypernociception, cell influx, vascular permeability, myeloperoxidase activity, nitrite levels, and histological changes were measured in TMJ lavages or tissues at selected time points. These parameters were also evaluated after treatment with the nitric oxide synthase (NOS) inhibitors L-NAME or 1400 W. Results. Zymosan-induced TMJ arthritis caused a time-dependent leucocyte migration, plasma extravasation, mechanical hypernociception, and neutrophil accumulation between 4 and 24 h. TMJ immunohistochemical analyses showed increased inducible NOS expression. Treatment with L-NAME or 1400 W inhibited these parameters. Conclusion. Zymosan-induced TMJ arthritis is a reproducible model that may be used to assess both the mechanisms underlying TMJ inflammation and the potential tools for therapies. Nitric oxide may participate in the inflammatory temporomandibular dysfunction mechanisms.
format Text
id pubmed-3022228
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-30222282011-01-27 Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils Chaves, Hellíada Vasconcelos Ribeiro, Ronaldo de Albuquerque de Souza, André Mattos Brito Silva, Antonio Alfredo Rodrigues e Gomes, Antoniella Souza Vale, Mariana Lima Bezerra, Mirna Marques Brito, Gerly Anne de Castro J Biomed Biotechnol Research Article Aims. To establish a new model of zymosan-induced temporomandibular joint (TMJ) arthritis in the rat and to investigate the role of nitric oxide. Methods. Inflammation was induced by an intra-articular injection of zymosan into the left TMJ. Mechanical hypernociception, cell influx, vascular permeability, myeloperoxidase activity, nitrite levels, and histological changes were measured in TMJ lavages or tissues at selected time points. These parameters were also evaluated after treatment with the nitric oxide synthase (NOS) inhibitors L-NAME or 1400 W. Results. Zymosan-induced TMJ arthritis caused a time-dependent leucocyte migration, plasma extravasation, mechanical hypernociception, and neutrophil accumulation between 4 and 24 h. TMJ immunohistochemical analyses showed increased inducible NOS expression. Treatment with L-NAME or 1400 W inhibited these parameters. Conclusion. Zymosan-induced TMJ arthritis is a reproducible model that may be used to assess both the mechanisms underlying TMJ inflammation and the potential tools for therapies. Nitric oxide may participate in the inflammatory temporomandibular dysfunction mechanisms. Hindawi Publishing Corporation 2011 2011-01-03 /pmc/articles/PMC3022228/ /pubmed/21274271 http://dx.doi.org/10.1155/2011/707985 Text en Copyright © 2011 Hellíada Vasconcelos Chaves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chaves, Hellíada Vasconcelos
Ribeiro, Ronaldo de Albuquerque
de Souza, André Mattos Brito
Silva, Antonio Alfredo Rodrigues e
Gomes, Antoniella Souza
Vale, Mariana Lima
Bezerra, Mirna Marques
Brito, Gerly Anne de Castro
Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title_full Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title_fullStr Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title_full_unstemmed Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title_short Experimental Model of Zymosan-Induced Arthritis in the Rat Temporomandibular Joint: Role of Nitric Oxide and Neutrophils
title_sort experimental model of zymosan-induced arthritis in the rat temporomandibular joint: role of nitric oxide and neutrophils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022228/
https://www.ncbi.nlm.nih.gov/pubmed/21274271
http://dx.doi.org/10.1155/2011/707985
work_keys_str_mv AT chaveshelliadavasconcelos experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT ribeiroronaldodealbuquerque experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT desouzaandremattosbrito experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT silvaantonioalfredorodriguese experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT gomesantoniellasouza experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT valemarianalima experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT bezerramirnamarques experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils
AT britogerlyannedecastro experimentalmodelofzymosaninducedarthritisintherattemporomandibularjointroleofnitricoxideandneutrophils