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An efficient Foxtail mosaic virus vector system with reduced environmental risk

BACKGROUND: Plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. The use of Agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsteri...

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Autores principales: Liu, Zun, Kearney, Christopher M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022558/
https://www.ncbi.nlm.nih.gov/pubmed/21162736
http://dx.doi.org/10.1186/1472-6750-10-88
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author Liu, Zun
Kearney, Christopher M
author_facet Liu, Zun
Kearney, Christopher M
author_sort Liu, Zun
collection PubMed
description BACKGROUND: Plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. The use of Agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsterile infiltration technique called "agroinoculation". With agroinoculation, a full length, systemically moving virus is no longer necessary for excellent protein yield, since the viral transgene is transcribed and replicates in every infiltrated cell. Viral genes may therefore be deleted to decrease the potential for accidental spread and persistence of the viral vector in the environment. RESULTS: In this study, both the coat protein (CP) and triple gene block (TGB) genetic segments were eliminated from Foxtail mosaic virus to create the "FECT" vector series, comprising a deletion of 29% of the genome. This viral vector is highly crippled and expresses little or no marker gene within the inoculated leaf. However, when co-agroinoculated with a silencing suppressor (p19 or HcPro), FECT expressed GFP at 40% total soluble protein in the tobacco host, Nicotiana benthamiana. The modified FoMV vector retained the full-length replicase ORF, the TGB1 subgenomic RNA leader sequence and either 0, 22 or 40 bases of TGB1 ORF (in vectors FECT0, FECT22 and FECT40, respectively). As well as N. benthamiana, infection of legumes was demonstrated. Despite many attempts, expression of GFP via syringe agroinoculation of various grass species was very low, reflecting the low Agrobacterium-mediated transformation rate of monocots. CONCLUSIONS: The FECT/40 vector expresses foreign genes at a very high level, and yet has a greatly reduced biohazard potential. It can form no virions and can effectively replicate only in a plant with suppressed silencing.
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spelling pubmed-30225582011-01-19 An efficient Foxtail mosaic virus vector system with reduced environmental risk Liu, Zun Kearney, Christopher M BMC Biotechnol Research Article BACKGROUND: Plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. The use of Agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsterile infiltration technique called "agroinoculation". With agroinoculation, a full length, systemically moving virus is no longer necessary for excellent protein yield, since the viral transgene is transcribed and replicates in every infiltrated cell. Viral genes may therefore be deleted to decrease the potential for accidental spread and persistence of the viral vector in the environment. RESULTS: In this study, both the coat protein (CP) and triple gene block (TGB) genetic segments were eliminated from Foxtail mosaic virus to create the "FECT" vector series, comprising a deletion of 29% of the genome. This viral vector is highly crippled and expresses little or no marker gene within the inoculated leaf. However, when co-agroinoculated with a silencing suppressor (p19 or HcPro), FECT expressed GFP at 40% total soluble protein in the tobacco host, Nicotiana benthamiana. The modified FoMV vector retained the full-length replicase ORF, the TGB1 subgenomic RNA leader sequence and either 0, 22 or 40 bases of TGB1 ORF (in vectors FECT0, FECT22 and FECT40, respectively). As well as N. benthamiana, infection of legumes was demonstrated. Despite many attempts, expression of GFP via syringe agroinoculation of various grass species was very low, reflecting the low Agrobacterium-mediated transformation rate of monocots. CONCLUSIONS: The FECT/40 vector expresses foreign genes at a very high level, and yet has a greatly reduced biohazard potential. It can form no virions and can effectively replicate only in a plant with suppressed silencing. BioMed Central 2010-12-16 /pmc/articles/PMC3022558/ /pubmed/21162736 http://dx.doi.org/10.1186/1472-6750-10-88 Text en Copyright ©2010 Liu and Kearney; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Zun
Kearney, Christopher M
An efficient Foxtail mosaic virus vector system with reduced environmental risk
title An efficient Foxtail mosaic virus vector system with reduced environmental risk
title_full An efficient Foxtail mosaic virus vector system with reduced environmental risk
title_fullStr An efficient Foxtail mosaic virus vector system with reduced environmental risk
title_full_unstemmed An efficient Foxtail mosaic virus vector system with reduced environmental risk
title_short An efficient Foxtail mosaic virus vector system with reduced environmental risk
title_sort efficient foxtail mosaic virus vector system with reduced environmental risk
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022558/
https://www.ncbi.nlm.nih.gov/pubmed/21162736
http://dx.doi.org/10.1186/1472-6750-10-88
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