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The maize ALDH protein superfamily: linking structural features to functional specificities
BACKGROUND: The completion of maize genome sequencing has resulted in the identification of a large number of uncharacterized genes. Gene annotation and functional characterization of gene products are important to uncover novel protein functionality. RESULTS: In this paper, we identify, and annotat...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022562/ https://www.ncbi.nlm.nih.gov/pubmed/21190582 http://dx.doi.org/10.1186/1472-6807-10-43 |
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author | Jimenez-Lopez, Jose C Gachomo, Emma W Seufferheld, Manfredo J Kotchoni, Simeon O |
author_facet | Jimenez-Lopez, Jose C Gachomo, Emma W Seufferheld, Manfredo J Kotchoni, Simeon O |
author_sort | Jimenez-Lopez, Jose C |
collection | PubMed |
description | BACKGROUND: The completion of maize genome sequencing has resulted in the identification of a large number of uncharacterized genes. Gene annotation and functional characterization of gene products are important to uncover novel protein functionality. RESULTS: In this paper, we identify, and annotate members of all the maize aldehyde dehydrogenase (ALDH) gene superfamily according to the revised nomenclature criteria developed by ALDH Gene Nomenclature Committee (AGNC). The maize genome contains 24 unique ALDH sequences encoding members of ten ALDH protein families including the previously identified male fertility restoration RF2A gene, which encodes a member of mitochondrial class 2 ALDHs. Using computational modeling analysis we report here the identification, the physico-chemical properties, and the amino acid residue analysis of a novel tunnel like cavity exclusively found in the maize sterility restorer protein, RF2A/ALDH2B2 by which this protein is suggested to bind variably long chain molecular ligands and/or potentially harmful molecules. CONCLUSIONS: Our finding indicates that maize ALDH superfamily is the most expanded of plant ALDHs ever characterized, and the mitochondrial maize RF2A/ALDH2B2 is the only plant ALDH that harbors a newly defined pocket/cavity with suggested functional specificity. |
format | Text |
id | pubmed-3022562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30225622011-01-19 The maize ALDH protein superfamily: linking structural features to functional specificities Jimenez-Lopez, Jose C Gachomo, Emma W Seufferheld, Manfredo J Kotchoni, Simeon O BMC Struct Biol Research Article BACKGROUND: The completion of maize genome sequencing has resulted in the identification of a large number of uncharacterized genes. Gene annotation and functional characterization of gene products are important to uncover novel protein functionality. RESULTS: In this paper, we identify, and annotate members of all the maize aldehyde dehydrogenase (ALDH) gene superfamily according to the revised nomenclature criteria developed by ALDH Gene Nomenclature Committee (AGNC). The maize genome contains 24 unique ALDH sequences encoding members of ten ALDH protein families including the previously identified male fertility restoration RF2A gene, which encodes a member of mitochondrial class 2 ALDHs. Using computational modeling analysis we report here the identification, the physico-chemical properties, and the amino acid residue analysis of a novel tunnel like cavity exclusively found in the maize sterility restorer protein, RF2A/ALDH2B2 by which this protein is suggested to bind variably long chain molecular ligands and/or potentially harmful molecules. CONCLUSIONS: Our finding indicates that maize ALDH superfamily is the most expanded of plant ALDHs ever characterized, and the mitochondrial maize RF2A/ALDH2B2 is the only plant ALDH that harbors a newly defined pocket/cavity with suggested functional specificity. BioMed Central 2010-12-29 /pmc/articles/PMC3022562/ /pubmed/21190582 http://dx.doi.org/10.1186/1472-6807-10-43 Text en Copyright ©2010 Jimenez-Lopez et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jimenez-Lopez, Jose C Gachomo, Emma W Seufferheld, Manfredo J Kotchoni, Simeon O The maize ALDH protein superfamily: linking structural features to functional specificities |
title | The maize ALDH protein superfamily: linking structural features to functional specificities |
title_full | The maize ALDH protein superfamily: linking structural features to functional specificities |
title_fullStr | The maize ALDH protein superfamily: linking structural features to functional specificities |
title_full_unstemmed | The maize ALDH protein superfamily: linking structural features to functional specificities |
title_short | The maize ALDH protein superfamily: linking structural features to functional specificities |
title_sort | maize aldh protein superfamily: linking structural features to functional specificities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022562/ https://www.ncbi.nlm.nih.gov/pubmed/21190582 http://dx.doi.org/10.1186/1472-6807-10-43 |
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