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The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008

BACKGROUND: Streptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associ...

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Autores principales: Hoa, Ngo T, Chieu, Tran TB, Nghia, Ho DT, Mai, Nguyen TH, Anh, Pham H, Wolbers, Marcel, Baker, Stephen, Campbell, James I, Chau, Nguyen VV, Hien, Tran T, Farrar, Jeremy, Schultsz, Constance
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022717/
https://www.ncbi.nlm.nih.gov/pubmed/21208459
http://dx.doi.org/10.1186/1471-2334-11-6
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author Hoa, Ngo T
Chieu, Tran TB
Nghia, Ho DT
Mai, Nguyen TH
Anh, Pham H
Wolbers, Marcel
Baker, Stephen
Campbell, James I
Chau, Nguyen VV
Hien, Tran T
Farrar, Jeremy
Schultsz, Constance
author_facet Hoa, Ngo T
Chieu, Tran TB
Nghia, Ho DT
Mai, Nguyen TH
Anh, Pham H
Wolbers, Marcel
Baker, Stephen
Campbell, James I
Chau, Nguyen VV
Hien, Tran T
Farrar, Jeremy
Schultsz, Constance
author_sort Hoa, Ngo T
collection PubMed
description BACKGROUND: Streptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associated resistance determinants in S. suis isolated from patients with meningitis in southern Vietnam. METHODS: S. suis strains isolated between 1997 and 2008 were investigated for their susceptibility to six antimicrobial agents. Strains were screened for the presence and expression of tetracycline and erythromycin resistance determinants and the association of tet(M) genes with Tn916- like transposons. The localization of tetracycline resistance gene tet(L) was determined by pulse field gel electrophoresis and Southern blotting. RESULTS: We observed a significant increase in resistance to tetracycline and chloramphenicol, which was concurrent with an increase in multi-drug resistance. In tetracycline resistance strains, we identified tet(M), tet(O), tet(W) and tet(L) and confirmed their expression. All tet(M) genes were associated with a Tn916-like transposon. The co-expression of tet(L) and other tetracycline resistance gene(s) encoding for ribosomal protection protein(s) was only detected in strains with a minimum inhibitory concentration (MIC) of tetracycline of ≥ 64 mg/L CONCLUSIONS: We demonstrated that multi-drug resistance in S. suis causing disease in humans in southern Vietnam has increased over the 11-year period studied. We report the presence and expression of tet(L) in S. suis strains and our data suggest that co-expression of multiple genes encoding distinct mechanism is required for an MIC ≥ 64 mg/L to tetracycline.
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spelling pubmed-30227172011-01-19 The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008 Hoa, Ngo T Chieu, Tran TB Nghia, Ho DT Mai, Nguyen TH Anh, Pham H Wolbers, Marcel Baker, Stephen Campbell, James I Chau, Nguyen VV Hien, Tran T Farrar, Jeremy Schultsz, Constance BMC Infect Dis Research Article BACKGROUND: Streptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associated resistance determinants in S. suis isolated from patients with meningitis in southern Vietnam. METHODS: S. suis strains isolated between 1997 and 2008 were investigated for their susceptibility to six antimicrobial agents. Strains were screened for the presence and expression of tetracycline and erythromycin resistance determinants and the association of tet(M) genes with Tn916- like transposons. The localization of tetracycline resistance gene tet(L) was determined by pulse field gel electrophoresis and Southern blotting. RESULTS: We observed a significant increase in resistance to tetracycline and chloramphenicol, which was concurrent with an increase in multi-drug resistance. In tetracycline resistance strains, we identified tet(M), tet(O), tet(W) and tet(L) and confirmed their expression. All tet(M) genes were associated with a Tn916-like transposon. The co-expression of tet(L) and other tetracycline resistance gene(s) encoding for ribosomal protection protein(s) was only detected in strains with a minimum inhibitory concentration (MIC) of tetracycline of ≥ 64 mg/L CONCLUSIONS: We demonstrated that multi-drug resistance in S. suis causing disease in humans in southern Vietnam has increased over the 11-year period studied. We report the presence and expression of tet(L) in S. suis strains and our data suggest that co-expression of multiple genes encoding distinct mechanism is required for an MIC ≥ 64 mg/L to tetracycline. BioMed Central 2011-01-06 /pmc/articles/PMC3022717/ /pubmed/21208459 http://dx.doi.org/10.1186/1471-2334-11-6 Text en Copyright ©2011 Hoa et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hoa, Ngo T
Chieu, Tran TB
Nghia, Ho DT
Mai, Nguyen TH
Anh, Pham H
Wolbers, Marcel
Baker, Stephen
Campbell, James I
Chau, Nguyen VV
Hien, Tran T
Farrar, Jeremy
Schultsz, Constance
The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title_full The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title_fullStr The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title_full_unstemmed The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title_short The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008
title_sort antimicrobial resistance patterns and associated determinants in streptococcus suis isolated from humans in southern vietnam, 1997-2008
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022717/
https://www.ncbi.nlm.nih.gov/pubmed/21208459
http://dx.doi.org/10.1186/1471-2334-11-6
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