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The Role of Catalase in Pulmonary Fibrosis

BACKGROUND: Catalase is preferentially expressed in bronchiolar and alveolar epithelial cells, and acts as an endogenous antioxidant enzyme in normal lungs. We thus postulated epithelial damage would be associated with a functional deficiency of catalase during the development of lung fibrosis. METH...

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Autores principales: Odajima, Nao, Betsuyaku, Tomoko, Nagai, Katsura, Moriyama, Chinatsu, Wang, Da-Hong, Takigawa, Tomoko, Ogino, Keiki, Nishimura, Masaharu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022724/
https://www.ncbi.nlm.nih.gov/pubmed/21190578
http://dx.doi.org/10.1186/1465-9921-11-183
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author Odajima, Nao
Betsuyaku, Tomoko
Nagai, Katsura
Moriyama, Chinatsu
Wang, Da-Hong
Takigawa, Tomoko
Ogino, Keiki
Nishimura, Masaharu
author_facet Odajima, Nao
Betsuyaku, Tomoko
Nagai, Katsura
Moriyama, Chinatsu
Wang, Da-Hong
Takigawa, Tomoko
Ogino, Keiki
Nishimura, Masaharu
author_sort Odajima, Nao
collection PubMed
description BACKGROUND: Catalase is preferentially expressed in bronchiolar and alveolar epithelial cells, and acts as an endogenous antioxidant enzyme in normal lungs. We thus postulated epithelial damage would be associated with a functional deficiency of catalase during the development of lung fibrosis. METHODS: The present study evaluates the expression of catalase mRNA and protein in human interstitial pneumonias and in mouse bleomycin-induced lung injury. We examined the degree of bleomycin-induced inflammation and fibrosis in the mice with lowered catalase activity. RESULTS: In humans, catalase was decreased at the levels of activity, protein content and mRNA expression in fibrotic lungs (n = 12) compared to control lungs (n = 10). Immunohistochemistry revealed a decrease in catalase in bronchiolar epithelium and abnormal re-epithelialization in fibrotic areas. In C57BL/6J mice, catalase activity was suppressed along with downregulation of catalase mRNA in whole lung homogenates after bleomycin administration. In acatalasemic mice, neutrophilic inflammation was prolonged until 14 days, and there was a higher degree of lung fibrosis in association with a higher level of transforming growth factor-β expression and total collagen content following bleomycin treatment compared to wild-type mice. CONCLUSIONS: Taken together, these findings demonstrate diminished catalase expression and activity in human pulmonary fibrosis and suggest the protective role of catalase against bleomycin-induced inflammation and subsequent fibrosis.
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spelling pubmed-30227242011-01-19 The Role of Catalase in Pulmonary Fibrosis Odajima, Nao Betsuyaku, Tomoko Nagai, Katsura Moriyama, Chinatsu Wang, Da-Hong Takigawa, Tomoko Ogino, Keiki Nishimura, Masaharu Respir Res Research BACKGROUND: Catalase is preferentially expressed in bronchiolar and alveolar epithelial cells, and acts as an endogenous antioxidant enzyme in normal lungs. We thus postulated epithelial damage would be associated with a functional deficiency of catalase during the development of lung fibrosis. METHODS: The present study evaluates the expression of catalase mRNA and protein in human interstitial pneumonias and in mouse bleomycin-induced lung injury. We examined the degree of bleomycin-induced inflammation and fibrosis in the mice with lowered catalase activity. RESULTS: In humans, catalase was decreased at the levels of activity, protein content and mRNA expression in fibrotic lungs (n = 12) compared to control lungs (n = 10). Immunohistochemistry revealed a decrease in catalase in bronchiolar epithelium and abnormal re-epithelialization in fibrotic areas. In C57BL/6J mice, catalase activity was suppressed along with downregulation of catalase mRNA in whole lung homogenates after bleomycin administration. In acatalasemic mice, neutrophilic inflammation was prolonged until 14 days, and there was a higher degree of lung fibrosis in association with a higher level of transforming growth factor-β expression and total collagen content following bleomycin treatment compared to wild-type mice. CONCLUSIONS: Taken together, these findings demonstrate diminished catalase expression and activity in human pulmonary fibrosis and suggest the protective role of catalase against bleomycin-induced inflammation and subsequent fibrosis. BioMed Central 2010 2010-12-29 /pmc/articles/PMC3022724/ /pubmed/21190578 http://dx.doi.org/10.1186/1465-9921-11-183 Text en Copyright ©2010 Odajima et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Odajima, Nao
Betsuyaku, Tomoko
Nagai, Katsura
Moriyama, Chinatsu
Wang, Da-Hong
Takigawa, Tomoko
Ogino, Keiki
Nishimura, Masaharu
The Role of Catalase in Pulmonary Fibrosis
title The Role of Catalase in Pulmonary Fibrosis
title_full The Role of Catalase in Pulmonary Fibrosis
title_fullStr The Role of Catalase in Pulmonary Fibrosis
title_full_unstemmed The Role of Catalase in Pulmonary Fibrosis
title_short The Role of Catalase in Pulmonary Fibrosis
title_sort role of catalase in pulmonary fibrosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3022724/
https://www.ncbi.nlm.nih.gov/pubmed/21190578
http://dx.doi.org/10.1186/1465-9921-11-183
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