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Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire

Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor...

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Autores principales: Viret, Christophe, Lamare, Camille, Guiraud, Martine, Fazilleau, Nicolas, Bour, Agathe, Malissen, Bernard, Carrier, Alice, Guerder, Sylvie
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023141/
https://www.ncbi.nlm.nih.gov/pubmed/21173102
http://dx.doi.org/10.1084/jem.20100027
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author Viret, Christophe
Lamare, Camille
Guiraud, Martine
Fazilleau, Nicolas
Bour, Agathe
Malissen, Bernard
Carrier, Alice
Guerder, Sylvie
author_facet Viret, Christophe
Lamare, Camille
Guiraud, Martine
Fazilleau, Nicolas
Bour, Agathe
Malissen, Bernard
Carrier, Alice
Guerder, Sylvie
author_sort Viret, Christophe
collection PubMed
description Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor (TCR) repertoire, the development of CD4 T cells expressing the OTII and Marilyn transgenic TCRs is impaired in TSSP-deficient mice. In this study, we assess the role of TSSP in shaping the functional endogenous polyclonal CD4 T cell repertoire by analyzing the response of TSSP-deficient mice to several protein antigens (Ags). Although TSSP-deficient mice responded normally to most of the Ags tested, they responded poorly to hen egg lysozyme (HEL). The impaired CD4 T cell response of TSSP-deficient mice to HEL correlated with significant alteration of the dominant TCR-β chain repertoire expressed by HEL-specific CD4 T cells, suggesting that TSSP is necessary for the intrathymic development of cells expressing these TCRs. Thus, TSSP contributes to the diversification of the functional endogenous CD4 T cell TCR repertoire in the thymus.
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spelling pubmed-30231412011-07-17 Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire Viret, Christophe Lamare, Camille Guiraud, Martine Fazilleau, Nicolas Bour, Agathe Malissen, Bernard Carrier, Alice Guerder, Sylvie J Exp Med Brief Definitive Report Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor (TCR) repertoire, the development of CD4 T cells expressing the OTII and Marilyn transgenic TCRs is impaired in TSSP-deficient mice. In this study, we assess the role of TSSP in shaping the functional endogenous polyclonal CD4 T cell repertoire by analyzing the response of TSSP-deficient mice to several protein antigens (Ags). Although TSSP-deficient mice responded normally to most of the Ags tested, they responded poorly to hen egg lysozyme (HEL). The impaired CD4 T cell response of TSSP-deficient mice to HEL correlated with significant alteration of the dominant TCR-β chain repertoire expressed by HEL-specific CD4 T cells, suggesting that TSSP is necessary for the intrathymic development of cells expressing these TCRs. Thus, TSSP contributes to the diversification of the functional endogenous CD4 T cell TCR repertoire in the thymus. The Rockefeller University Press 2011-01-17 /pmc/articles/PMC3023141/ /pubmed/21173102 http://dx.doi.org/10.1084/jem.20100027 Text en © 2011 Viret et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Brief Definitive Report
Viret, Christophe
Lamare, Camille
Guiraud, Martine
Fazilleau, Nicolas
Bour, Agathe
Malissen, Bernard
Carrier, Alice
Guerder, Sylvie
Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title_full Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title_fullStr Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title_full_unstemmed Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title_short Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
title_sort thymus-specific serine protease contributes to the diversification of the functional endogenous cd4 t cell receptor repertoire
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023141/
https://www.ncbi.nlm.nih.gov/pubmed/21173102
http://dx.doi.org/10.1084/jem.20100027
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