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Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire
Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023141/ https://www.ncbi.nlm.nih.gov/pubmed/21173102 http://dx.doi.org/10.1084/jem.20100027 |
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author | Viret, Christophe Lamare, Camille Guiraud, Martine Fazilleau, Nicolas Bour, Agathe Malissen, Bernard Carrier, Alice Guerder, Sylvie |
author_facet | Viret, Christophe Lamare, Camille Guiraud, Martine Fazilleau, Nicolas Bour, Agathe Malissen, Bernard Carrier, Alice Guerder, Sylvie |
author_sort | Viret, Christophe |
collection | PubMed |
description | Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor (TCR) repertoire, the development of CD4 T cells expressing the OTII and Marilyn transgenic TCRs is impaired in TSSP-deficient mice. In this study, we assess the role of TSSP in shaping the functional endogenous polyclonal CD4 T cell repertoire by analyzing the response of TSSP-deficient mice to several protein antigens (Ags). Although TSSP-deficient mice responded normally to most of the Ags tested, they responded poorly to hen egg lysozyme (HEL). The impaired CD4 T cell response of TSSP-deficient mice to HEL correlated with significant alteration of the dominant TCR-β chain repertoire expressed by HEL-specific CD4 T cells, suggesting that TSSP is necessary for the intrathymic development of cells expressing these TCRs. Thus, TSSP contributes to the diversification of the functional endogenous CD4 T cell TCR repertoire in the thymus. |
format | Text |
id | pubmed-3023141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30231412011-07-17 Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire Viret, Christophe Lamare, Camille Guiraud, Martine Fazilleau, Nicolas Bour, Agathe Malissen, Bernard Carrier, Alice Guerder, Sylvie J Exp Med Brief Definitive Report Thymus-specific serine protease (TSSP) is a novel protease that may contribute to the generation of the peptide repertoire presented by MHC class II molecules in the thymus. Although TSSP deficiency has no quantitative impact on the development of CD4 T cells expressing a polyclonal T cell receptor (TCR) repertoire, the development of CD4 T cells expressing the OTII and Marilyn transgenic TCRs is impaired in TSSP-deficient mice. In this study, we assess the role of TSSP in shaping the functional endogenous polyclonal CD4 T cell repertoire by analyzing the response of TSSP-deficient mice to several protein antigens (Ags). Although TSSP-deficient mice responded normally to most of the Ags tested, they responded poorly to hen egg lysozyme (HEL). The impaired CD4 T cell response of TSSP-deficient mice to HEL correlated with significant alteration of the dominant TCR-β chain repertoire expressed by HEL-specific CD4 T cells, suggesting that TSSP is necessary for the intrathymic development of cells expressing these TCRs. Thus, TSSP contributes to the diversification of the functional endogenous CD4 T cell TCR repertoire in the thymus. The Rockefeller University Press 2011-01-17 /pmc/articles/PMC3023141/ /pubmed/21173102 http://dx.doi.org/10.1084/jem.20100027 Text en © 2011 Viret et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Brief Definitive Report Viret, Christophe Lamare, Camille Guiraud, Martine Fazilleau, Nicolas Bour, Agathe Malissen, Bernard Carrier, Alice Guerder, Sylvie Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title | Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title_full | Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title_fullStr | Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title_full_unstemmed | Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title_short | Thymus-specific serine protease contributes to the diversification of the functional endogenous CD4 T cell receptor repertoire |
title_sort | thymus-specific serine protease contributes to the diversification of the functional endogenous cd4 t cell receptor repertoire |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023141/ https://www.ncbi.nlm.nih.gov/pubmed/21173102 http://dx.doi.org/10.1084/jem.20100027 |
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