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Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells

BACKGROUND: Quantification of transcripts, proteins, or metabolites is straightforward when the factor used to normalize these values remains constant between samples. However, normalization factors often vary among samples and thus must be developed for each new analytical method. RESULTS: We demon...

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Detalles Bibliográficos
Autores principales: Shimada, Hiroshi, Obayashi, Takeshi, Takahashi, Naoki, Matsui, Minami, Sakamoto, Atsushi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023742/
https://www.ncbi.nlm.nih.gov/pubmed/21190547
http://dx.doi.org/10.1186/1746-4811-6-29