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Stabilization by Fusion to the C-terminus of Hyperthermophile Sulfolobus tokodaii RNase HI: A Possibility of Protein Stabilization Tag
RNase HI from the hyperthermophile Sulfolobus tokodaii (Sto-RNase HI) is stabilized by its C-terminal residues. In this work, the stabilization effect of the Sto-RNase HI C-terminal residues was investigated in detail by thermodynamic measurements of the stability of variants lacking the disulfide b...
Autores principales: | Takano, Kazufumi, Okamoto, Tomohiro, Okada, Jun, Tanaka, Shun-ichi, Angkawidjaja, Clement, Koga, Yuichi, Kanaya, Shigenori |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023800/ https://www.ncbi.nlm.nih.gov/pubmed/21283826 http://dx.doi.org/10.1371/journal.pone.0016226 |
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