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Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq
We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a m...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Microbiology
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023915/ https://www.ncbi.nlm.nih.gov/pubmed/21253457 http://dx.doi.org/10.1128/mBio.00315-10 |