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Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq

We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a m...

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Detalles Bibliográficos
Autores principales:	Gallagher, Larry A., Shendure, Jay, Manoil, Colin
Formato:	Texto
Lenguaje:	English
Publicado:	American Society of Microbiology 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3023915/ https://www.ncbi.nlm.nih.gov/pubmed/21253457 http://dx.doi.org/10.1128/mBio.00315-10

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