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Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation

Objective: Impaired microcirculation is one of the most important factors in delayed wound healing. The aim of the study was to investigate the influence of chemical and surgical interruption of sympathetic nerve fibers and α- and β-receptors blockers on muscular microcirculation. Methods: The exper...

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Autores principales: Homann, Heinz H., Hirsch, Tobias, Steinau, H.U., Muehlberger, Thomas, Moll, Wibke, Lehnhardt, Marcus, Goertz, Ole
Formato: Texto
Lenguaje:English
Publicado: Open Science Company, LLC 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024898/
https://www.ncbi.nlm.nih.gov/pubmed/21283734
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author Homann, Heinz H.
Hirsch, Tobias
Steinau, H.U.
Muehlberger, Thomas
Moll, Wibke
Lehnhardt, Marcus
Goertz, Ole
author_facet Homann, Heinz H.
Hirsch, Tobias
Steinau, H.U.
Muehlberger, Thomas
Moll, Wibke
Lehnhardt, Marcus
Goertz, Ole
author_sort Homann, Heinz H.
collection PubMed
description Objective: Impaired microcirculation is one of the most important factors in delayed wound healing. The aim of the study was to investigate the influence of chemical and surgical interruption of sympathetic nerve fibers and α- and β-receptors blockers on muscular microcirculation. Methods: The experiment was performed on a standardized cremaster muscle model of male Wistar rats (n=51). Microcirculation was recorded via transillumination microscopy on each of the 4 test groups and in a control group before and after their respective treatments with one of the following: topical application of bupivacaine, metoprolol, phentolamine, or surgical denervation. The arteriolar diameter and functional capillary density (FCD) as parameter for tissue perfusion were assessed. Results: The α-blocker phentolamine was the only agent that caused a significant dilation of the arteriolar diameter (76.6 ± 6.9 vs 100.0 ± 12.0 µm). However, like bupivacaine, metoprolol, and the surgical sympathectomy, it did not improve FCD as a parameter for tissue perfusion. The strongest vasoconstriction (35.9 ± 4.3 vs 28.6 ± 4.0) and impairment of the FCD (10.0 ± 0.7 vs 4.1 ± 0.9) was induced by the β-blocker metoprolol. Conclusions: This study shows that phentolamine could be an agent for dilating arteriolar diameter, but it did not improve FCD. Whereas the other agents, including sympathectomy, did not alter arteriolar diameter, the β-blocker worsened both investigated parameters. Our results raise the question whether β-blockers negatively influence microcirculation. Therefore, further studies are needed to investigate the potential adverse effects of β-blockers on wound healing.
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spelling pubmed-30248982011-01-31 Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation Homann, Heinz H. Hirsch, Tobias Steinau, H.U. Muehlberger, Thomas Moll, Wibke Lehnhardt, Marcus Goertz, Ole Eplasty Journal Article Objective: Impaired microcirculation is one of the most important factors in delayed wound healing. The aim of the study was to investigate the influence of chemical and surgical interruption of sympathetic nerve fibers and α- and β-receptors blockers on muscular microcirculation. Methods: The experiment was performed on a standardized cremaster muscle model of male Wistar rats (n=51). Microcirculation was recorded via transillumination microscopy on each of the 4 test groups and in a control group before and after their respective treatments with one of the following: topical application of bupivacaine, metoprolol, phentolamine, or surgical denervation. The arteriolar diameter and functional capillary density (FCD) as parameter for tissue perfusion were assessed. Results: The α-blocker phentolamine was the only agent that caused a significant dilation of the arteriolar diameter (76.6 ± 6.9 vs 100.0 ± 12.0 µm). However, like bupivacaine, metoprolol, and the surgical sympathectomy, it did not improve FCD as a parameter for tissue perfusion. The strongest vasoconstriction (35.9 ± 4.3 vs 28.6 ± 4.0) and impairment of the FCD (10.0 ± 0.7 vs 4.1 ± 0.9) was induced by the β-blocker metoprolol. Conclusions: This study shows that phentolamine could be an agent for dilating arteriolar diameter, but it did not improve FCD. Whereas the other agents, including sympathectomy, did not alter arteriolar diameter, the β-blocker worsened both investigated parameters. Our results raise the question whether β-blockers negatively influence microcirculation. Therefore, further studies are needed to investigate the potential adverse effects of β-blockers on wound healing. Open Science Company, LLC 2011-01-20 /pmc/articles/PMC3024898/ /pubmed/21283734 Text en Copyright © 2011 The Author(s) http://creativecommons.org/licenses/by/2.0/ This is an open-access article whereby the authors retain copyright of the work. The article is distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Journal Article
Homann, Heinz H.
Hirsch, Tobias
Steinau, H.U.
Muehlberger, Thomas
Moll, Wibke
Lehnhardt, Marcus
Goertz, Ole
Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title_full Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title_fullStr Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title_full_unstemmed Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title_short Influence of Receptor Antagonists, Local Anesthetics, and Denervation on Microcirculation
title_sort influence of receptor antagonists, local anesthetics, and denervation on microcirculation
topic Journal Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3024898/
https://www.ncbi.nlm.nih.gov/pubmed/21283734
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