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Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress
AIM: The antioxidant effect of the methanol–methylene chloride extract of Terminalia glaucescens (Combretaceae) leaves was investigated in streptozotocin (STZ)-induced oxidative stress. METHODS: Oxidative stress was induced in mice by a daily dose of STZ (45 mg/kg body weight i.p.) for five days. Fr...
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Formato: | Texto |
Lenguaje: | English |
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Medknow Publications
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025144/ https://www.ncbi.nlm.nih.gov/pubmed/21279183 http://dx.doi.org/10.4103/0253-7613.45153 |
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author | Njomen, Guy Bertrand Sabas Nya Kamgang, René Oyono, Jean Louis Essame Njikam, Njifutie |
author_facet | Njomen, Guy Bertrand Sabas Nya Kamgang, René Oyono, Jean Louis Essame Njikam, Njifutie |
author_sort | Njomen, Guy Bertrand Sabas Nya |
collection | PubMed |
description | AIM: The antioxidant effect of the methanol–methylene chloride extract of Terminalia glaucescens (Combretaceae) leaves was investigated in streptozotocin (STZ)-induced oxidative stress. METHODS: Oxidative stress was induced in mice by a daily dose of STZ (45 mg/kg body weight i.p.) for five days. From day one, before STZ injection, normal and diabetic-test mice received an oral dose of the extract (100 or 300 mg/kg b.w.) daily. Plasma metabolites, lipid peroxidation, and antioxidant enzymes in the liver were assessed and gain in body weight recorded. RESULTS: In normal mice the plant extract reduced food and water intake, blood glucose and LDL-C level and body weight gain, did not affect the lipid peroxidation in the liver, while the antioxidant enzyme activities seemed increased. Blood glucose was decreased (P < 0.05) in normal mice treated with 300 mg/kg extract. Diabetic mice pretreated with 100 mg/kg extract as diabetic control mice (DC) showed significant (P < 0.001) body weight loss, polyphagia and polydipsia, high plasma glucose level, decrease in the liver catalase, peroxidase, and superoxide dismutase activities, and increase in lipid peroxidation. The HDL-C level was lowered (P < 0.05) whereas LDL-C increased. In 300 mg/kg extract-pretreated diabetic mice the extract prevented body weight loss, increase of blood glucose level, lipid peroxidation in liver, food and water intake, and lowering of plasma HDL-C level and liver antioxidants; this extract prevented LDL-C level increase. CONCLUSION: These results indicate that T. glaucescens protects against STZ-induced oxidative stress and could thus explain its traditional use for diabetes and obesity treatment or management. |
format | Text |
id | pubmed-3025144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-30251442011-01-28 Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress Njomen, Guy Bertrand Sabas Nya Kamgang, René Oyono, Jean Louis Essame Njikam, Njifutie Indian J Pharmacol Research Article AIM: The antioxidant effect of the methanol–methylene chloride extract of Terminalia glaucescens (Combretaceae) leaves was investigated in streptozotocin (STZ)-induced oxidative stress. METHODS: Oxidative stress was induced in mice by a daily dose of STZ (45 mg/kg body weight i.p.) for five days. From day one, before STZ injection, normal and diabetic-test mice received an oral dose of the extract (100 or 300 mg/kg b.w.) daily. Plasma metabolites, lipid peroxidation, and antioxidant enzymes in the liver were assessed and gain in body weight recorded. RESULTS: In normal mice the plant extract reduced food and water intake, blood glucose and LDL-C level and body weight gain, did not affect the lipid peroxidation in the liver, while the antioxidant enzyme activities seemed increased. Blood glucose was decreased (P < 0.05) in normal mice treated with 300 mg/kg extract. Diabetic mice pretreated with 100 mg/kg extract as diabetic control mice (DC) showed significant (P < 0.001) body weight loss, polyphagia and polydipsia, high plasma glucose level, decrease in the liver catalase, peroxidase, and superoxide dismutase activities, and increase in lipid peroxidation. The HDL-C level was lowered (P < 0.05) whereas LDL-C increased. In 300 mg/kg extract-pretreated diabetic mice the extract prevented body weight loss, increase of blood glucose level, lipid peroxidation in liver, food and water intake, and lowering of plasma HDL-C level and liver antioxidants; this extract prevented LDL-C level increase. CONCLUSION: These results indicate that T. glaucescens protects against STZ-induced oxidative stress and could thus explain its traditional use for diabetes and obesity treatment or management. Medknow Publications 2008 /pmc/articles/PMC3025144/ /pubmed/21279183 http://dx.doi.org/10.4103/0253-7613.45153 Text en © Indian Journal of Pharmacology http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Njomen, Guy Bertrand Sabas Nya Kamgang, René Oyono, Jean Louis Essame Njikam, Njifutie Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title | Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title_full | Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title_fullStr | Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title_full_unstemmed | Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title_short | Antioxidant potential of the methanol–methylene chloride extract of Terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
title_sort | antioxidant potential of the methanol–methylene chloride extract of terminalia glaucescens leaves on mice liver in streptozotocin-induced stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025144/ https://www.ncbi.nlm.nih.gov/pubmed/21279183 http://dx.doi.org/10.4103/0253-7613.45153 |
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