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Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Homing endonucleases recognize long target DNA sequences generating an accurate double-strand break that promotes gene targeting through homologous recombination. We have modified the homodimeric I-CreI endonuclease through protein engineering to target a specific DNA sequence within the human RAG1...

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Detalles Bibliográficos
Autores principales:	Muñoz, Inés G., Prieto, Jesús, Subramanian, Sunita, Coloma, Javier, Redondo, Pilar, Villate, Maider, Merino, Nekane, Marenchino, Marco, D'Abramo, Marco, Gervasio, Francesco L., Grizot, Sylvestre, Daboussi, Fayza, Smith, Julianne, Chion-Sotinel, Isabelle, Pâques, Frédéric, Duchateau, Philippe, Alibés, Andreu, Stricher, François, Serrano, Luis, Blanco, Francisco J., Montoya, Guillermo
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025557/ https://www.ncbi.nlm.nih.gov/pubmed/20846960 http://dx.doi.org/10.1093/nar/gkq801

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