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Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules

X-ray radiation damage propagation is explored for hydrated starch granules in order to reduce the step resolution in raster-microdiffraction experiments to the nanometre range. Radiation damage was induced by synchrotron radiation microbeams of 5, 1 and 0.3 µm size with ∼0.1 nm wavelength in B-type...

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Autores principales: Riekel, C., Burghammer, M., Davies, R. J., Di Cola, E., König, C., Lemke, H.T., Putaux, J.-L., Schöder, S.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025595/
https://www.ncbi.nlm.nih.gov/pubmed/20975219
http://dx.doi.org/10.1107/S0909049510028335
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author Riekel, C.
Burghammer, M.
Davies, R. J.
Di Cola, E.
König, C.
Lemke, H.T.
Putaux, J.-L.
Schöder, S.
author_facet Riekel, C.
Burghammer, M.
Davies, R. J.
Di Cola, E.
König, C.
Lemke, H.T.
Putaux, J.-L.
Schöder, S.
author_sort Riekel, C.
collection PubMed
description X-ray radiation damage propagation is explored for hydrated starch granules in order to reduce the step resolution in raster-microdiffraction experiments to the nanometre range. Radiation damage was induced by synchrotron radiation microbeams of 5, 1 and 0.3 µm size with ∼0.1 nm wavelength in B-type potato, Canna edulis and Phajus grandifolius starch granules. A total loss of crystallinity of granules immersed in water was found at a dose of ∼1.3 photons nm(−3). The temperature dependence of radiation damage suggests that primary radiation damage prevails up to about 120 K while secondary radiation damage becomes effective at higher temperatures. Primary radiation damage remains confined to the beam track at 100 K. Propagation of radiation damage beyond the beam track at room temperature is assumed to be due to reactive species generated principally by water radiolysis induced by photoelectrons. By careful dose selection during data collection, raster scans with 500 nm step-resolution could be performed for granules immersed in water.
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spelling pubmed-30255952011-01-25 Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules Riekel, C. Burghammer, M. Davies, R. J. Di Cola, E. König, C. Lemke, H.T. Putaux, J.-L. Schöder, S. J Synchrotron Radiat Research Papers X-ray radiation damage propagation is explored for hydrated starch granules in order to reduce the step resolution in raster-microdiffraction experiments to the nanometre range. Radiation damage was induced by synchrotron radiation microbeams of 5, 1 and 0.3 µm size with ∼0.1 nm wavelength in B-type potato, Canna edulis and Phajus grandifolius starch granules. A total loss of crystallinity of granules immersed in water was found at a dose of ∼1.3 photons nm(−3). The temperature dependence of radiation damage suggests that primary radiation damage prevails up to about 120 K while secondary radiation damage becomes effective at higher temperatures. Primary radiation damage remains confined to the beam track at 100 K. Propagation of radiation damage beyond the beam track at room temperature is assumed to be due to reactive species generated principally by water radiolysis induced by photoelectrons. By careful dose selection during data collection, raster scans with 500 nm step-resolution could be performed for granules immersed in water. International Union of Crystallography 2010-11-01 2010-09-03 /pmc/articles/PMC3025595/ /pubmed/20975219 http://dx.doi.org/10.1107/S0909049510028335 Text en © C. Riekel et al. 2010 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Riekel, C.
Burghammer, M.
Davies, R. J.
Di Cola, E.
König, C.
Lemke, H.T.
Putaux, J.-L.
Schöder, S.
Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title_full Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title_fullStr Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title_full_unstemmed Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title_short Raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
title_sort raster microdiffraction with synchrotron radiation of hydrated biopolymers with nanometre step-resolution: case study of starch granules
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025595/
https://www.ncbi.nlm.nih.gov/pubmed/20975219
http://dx.doi.org/10.1107/S0909049510028335
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