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The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis

BACKGROUND: Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mec...

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Detalles Bibliográficos
Autores principales:	Balart, Josep, Pueyo, Gemma, de Llobet, Lara I, Baro, Marta, Sole, Xavi, Marin, Susanna, Casanovas, Oriol, Mesia, Ricard, Capella, Gabriel
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025872/ https://www.ncbi.nlm.nih.gov/pubmed/21235815 http://dx.doi.org/10.1186/1748-717X-6-6

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