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Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality

BACKGROUND: The increasingly narrow genetic background characteristic of modern crop germplasm presents a challenge for the breeding of cultivars that require adaptation to the anticipated change in climate. Thus, high priority research aims at the identification of relevant allelic variation presen...

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Autores principales: Worch, Sebastian, Rajesh, Kalladan, Harshavardhan, Vokkaliga T, Pietsch, Christof, Korzun, Viktor, Kuntze, Lissy, Börner, Andreas, Wobus, Ulrich, Röder, Marion S, Sreenivasulu, Nese
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025944/
https://www.ncbi.nlm.nih.gov/pubmed/21205309
http://dx.doi.org/10.1186/1471-2229-11-1
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author Worch, Sebastian
Rajesh, Kalladan
Harshavardhan, Vokkaliga T
Pietsch, Christof
Korzun, Viktor
Kuntze, Lissy
Börner, Andreas
Wobus, Ulrich
Röder, Marion S
Sreenivasulu, Nese
author_facet Worch, Sebastian
Rajesh, Kalladan
Harshavardhan, Vokkaliga T
Pietsch, Christof
Korzun, Viktor
Kuntze, Lissy
Börner, Andreas
Wobus, Ulrich
Röder, Marion S
Sreenivasulu, Nese
author_sort Worch, Sebastian
collection PubMed
description BACKGROUND: The increasingly narrow genetic background characteristic of modern crop germplasm presents a challenge for the breeding of cultivars that require adaptation to the anticipated change in climate. Thus, high priority research aims at the identification of relevant allelic variation present both in the crop itself as well as in its progenitors. This study is based on the characterization of genetic variation in barley, with a view to enhancing its response to terminal drought stress. RESULTS: The expression patterns of drought regulated genes were monitored during plant ontogeny, mapped and the location of these genes was incorporated into a comprehensive barley SNP linkage map. Haplotypes within a set of 17 starch biosynthesis/degradation genes were defined, and a particularly high level of haplotype variation was uncovered in the genes encoding sucrose synthase (types I and II) and starch synthase. The ability of a panel of 50 barley accessions to maintain grain starch content under terminal drought conditions was explored. CONCLUSION: The linkage/expression map is an informative resource in the context of characterizing the response of barley to drought stress. The high level of haplotype variation among starch biosynthesis/degradation genes in the progenitors of cultivated barley shows that domestication and breeding have greatly eroded their allelic diversity in current elite cultivars. Prospective association analysis based on core drought-regulated genes may simplify the process of identifying favourable alleles, and help to understand the genetic basis of the response to terminal drought.
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spelling pubmed-30259442011-01-25 Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality Worch, Sebastian Rajesh, Kalladan Harshavardhan, Vokkaliga T Pietsch, Christof Korzun, Viktor Kuntze, Lissy Börner, Andreas Wobus, Ulrich Röder, Marion S Sreenivasulu, Nese BMC Plant Biol Research Article BACKGROUND: The increasingly narrow genetic background characteristic of modern crop germplasm presents a challenge for the breeding of cultivars that require adaptation to the anticipated change in climate. Thus, high priority research aims at the identification of relevant allelic variation present both in the crop itself as well as in its progenitors. This study is based on the characterization of genetic variation in barley, with a view to enhancing its response to terminal drought stress. RESULTS: The expression patterns of drought regulated genes were monitored during plant ontogeny, mapped and the location of these genes was incorporated into a comprehensive barley SNP linkage map. Haplotypes within a set of 17 starch biosynthesis/degradation genes were defined, and a particularly high level of haplotype variation was uncovered in the genes encoding sucrose synthase (types I and II) and starch synthase. The ability of a panel of 50 barley accessions to maintain grain starch content under terminal drought conditions was explored. CONCLUSION: The linkage/expression map is an informative resource in the context of characterizing the response of barley to drought stress. The high level of haplotype variation among starch biosynthesis/degradation genes in the progenitors of cultivated barley shows that domestication and breeding have greatly eroded their allelic diversity in current elite cultivars. Prospective association analysis based on core drought-regulated genes may simplify the process of identifying favourable alleles, and help to understand the genetic basis of the response to terminal drought. BioMed Central 2011-01-04 /pmc/articles/PMC3025944/ /pubmed/21205309 http://dx.doi.org/10.1186/1471-2229-11-1 Text en Copyright ©2011 Worch et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Worch, Sebastian
Rajesh, Kalladan
Harshavardhan, Vokkaliga T
Pietsch, Christof
Korzun, Viktor
Kuntze, Lissy
Börner, Andreas
Wobus, Ulrich
Röder, Marion S
Sreenivasulu, Nese
Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title_full Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title_fullStr Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title_full_unstemmed Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title_short Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
title_sort haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025944/
https://www.ncbi.nlm.nih.gov/pubmed/21205309
http://dx.doi.org/10.1186/1471-2229-11-1
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