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COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)

BACKGROUND: COX-2 is an enzyme isoform that catalyses the formation of prostanoids from arachidonic acid. An increased COX-2 gene expression is believed to participate in carcinogenesis. Recent studies have shown that COX-2 up-regulation is associated with the development of numerous neoplasms, incl...

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Autores principales: Krawczyk-Rusiecka, Kinga, Wojciechowska-Durczyńska, Katarzyna, Cyniak-Magierska, Anna, Adamczewski, Zbigniew, Gałecka, Elżbieta, Lewiński, Andrzej
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025973/
https://www.ncbi.nlm.nih.gov/pubmed/21214962
http://dx.doi.org/10.1186/1756-6614-4-3
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author Krawczyk-Rusiecka, Kinga
Wojciechowska-Durczyńska, Katarzyna
Cyniak-Magierska, Anna
Adamczewski, Zbigniew
Gałecka, Elżbieta
Lewiński, Andrzej
author_facet Krawczyk-Rusiecka, Kinga
Wojciechowska-Durczyńska, Katarzyna
Cyniak-Magierska, Anna
Adamczewski, Zbigniew
Gałecka, Elżbieta
Lewiński, Andrzej
author_sort Krawczyk-Rusiecka, Kinga
collection PubMed
description BACKGROUND: COX-2 is an enzyme isoform that catalyses the formation of prostanoids from arachidonic acid. An increased COX-2 gene expression is believed to participate in carcinogenesis. Recent studies have shown that COX-2 up-regulation is associated with the development of numerous neoplasms, including skin, colorectal, breast, lung, stomach, pancreas and liver cancers. COX-2 products stimulate endothelial cell proliferation and their overexpression has been demonstrated to be involved in the mechanism of decreased resistance to apoptosis. Suppressed angiogenesis was found in experimental animal studies as a consequence of null mutation of COX-2 gene in mice. Despite the role of COX-2 expression remains a subject of numerous studies, its participation in carcinogenesis or the thyroid cancer progression remains unclear. METHODS: Twenty three (23) patients with cytological diagnosis of PTC were evaluated. After FNAB examination, the needle was washed out with a lysis buffer and the obtained material was used for COX-2 expression estimation. Total RNA was isolated (RNeasy Micro Kit), and RT reactions were performed. β-actin was used as endogenous control. Relative COX-2 expression was assessed in real-time PCR reactions by an ABI PRISM 7500 Sequence Detection System, using the ΔΔC(T )method. RESULTS: COX-2 gene expression was higher in patients with PTC, when compared to specimens from patients with non-toxic nodular goitre (NTG). CONCLUSIONS: The preliminary results may indicate COX-2 role in thyroid cancer pathogenesis, however the observed variability in results among particular subjects requires additional clinical data and tumor progression analysis.
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spelling pubmed-30259732011-01-25 COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB) Krawczyk-Rusiecka, Kinga Wojciechowska-Durczyńska, Katarzyna Cyniak-Magierska, Anna Adamczewski, Zbigniew Gałecka, Elżbieta Lewiński, Andrzej Thyroid Res Research BACKGROUND: COX-2 is an enzyme isoform that catalyses the formation of prostanoids from arachidonic acid. An increased COX-2 gene expression is believed to participate in carcinogenesis. Recent studies have shown that COX-2 up-regulation is associated with the development of numerous neoplasms, including skin, colorectal, breast, lung, stomach, pancreas and liver cancers. COX-2 products stimulate endothelial cell proliferation and their overexpression has been demonstrated to be involved in the mechanism of decreased resistance to apoptosis. Suppressed angiogenesis was found in experimental animal studies as a consequence of null mutation of COX-2 gene in mice. Despite the role of COX-2 expression remains a subject of numerous studies, its participation in carcinogenesis or the thyroid cancer progression remains unclear. METHODS: Twenty three (23) patients with cytological diagnosis of PTC were evaluated. After FNAB examination, the needle was washed out with a lysis buffer and the obtained material was used for COX-2 expression estimation. Total RNA was isolated (RNeasy Micro Kit), and RT reactions were performed. β-actin was used as endogenous control. Relative COX-2 expression was assessed in real-time PCR reactions by an ABI PRISM 7500 Sequence Detection System, using the ΔΔC(T )method. RESULTS: COX-2 gene expression was higher in patients with PTC, when compared to specimens from patients with non-toxic nodular goitre (NTG). CONCLUSIONS: The preliminary results may indicate COX-2 role in thyroid cancer pathogenesis, however the observed variability in results among particular subjects requires additional clinical data and tumor progression analysis. BioMed Central 2011-01-10 /pmc/articles/PMC3025973/ /pubmed/21214962 http://dx.doi.org/10.1186/1756-6614-4-3 Text en Copyright ©2011 Krawczyk-Rusiecka et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Krawczyk-Rusiecka, Kinga
Wojciechowska-Durczyńska, Katarzyna
Cyniak-Magierska, Anna
Adamczewski, Zbigniew
Gałecka, Elżbieta
Lewiński, Andrzej
COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title_full COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title_fullStr COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title_full_unstemmed COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title_short COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB)
title_sort cox-2 expression in papillary thyroid carcinoma (ptc) in cytological material obtained by fine needle aspiration biopsy (fnab)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025973/
https://www.ncbi.nlm.nih.gov/pubmed/21214962
http://dx.doi.org/10.1186/1756-6614-4-3
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