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A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers

Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f(+)CD45(−)Thy1(−) cell fraction. However, these cells were a heterogeneous cell population containing various stages of differentiation. This study aimed to detect more immature...

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Autores principales: Konishi, Sayuri, Yasuchika, Kentaro, Ishii, Takamichi, Fukumitsu, Ken, Kamo, Naoko, Fujita, Naoya, Ikai, Iwao, Uemoto, Shinji
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026936/
https://www.ncbi.nlm.nih.gov/pubmed/21104040
http://dx.doi.org/10.1007/s11626-010-9354-7
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author Konishi, Sayuri
Yasuchika, Kentaro
Ishii, Takamichi
Fukumitsu, Ken
Kamo, Naoko
Fujita, Naoya
Ikai, Iwao
Uemoto, Shinji
author_facet Konishi, Sayuri
Yasuchika, Kentaro
Ishii, Takamichi
Fukumitsu, Ken
Kamo, Naoko
Fujita, Naoya
Ikai, Iwao
Uemoto, Shinji
author_sort Konishi, Sayuri
collection PubMed
description Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f(+)CD45(−)Thy1(−) cell fraction. However, these cells were a heterogeneous cell population containing various stages of differentiation. This study aimed to detect more immature HPCs, using a novel surface antigen, gp38. After the collagenase digestion of fetal livers harvested from E13.5 to E18.5 fetal mice, HPCs were obtained and divided into two subpopulations using flow cytometry: gp38-positive HPCs, and gp38-negative HPCs. Both types of HPCs were characterized by immunocytochemistry and RT-PCR. The proliferative activity was compared by BrdU incorporation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay. Furthermore, the comprehensive gene expression was investigated by DNA microarray. Both types of HPCs expressed alpha-fetoprotein. However, the gp38-positive HPCs derived from E13.5 fetal livers did not express albumin or cytokeratin 19, while the gp38-negative HPCs did. DNA microarray revealed that some genes related to the Wnt signal pathway were up-regulated in the gp38-positive HPCs. Furthermore, Wnt3a had a proliferative effect on the gp38-positive HPCs. In conclusion, the gp38-positive HPCs derived from fetal liver tissue until E13.5 could therefore be candidates for hepatic stem cells in the fetal liver.
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spelling pubmed-30269362011-02-22 A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers Konishi, Sayuri Yasuchika, Kentaro Ishii, Takamichi Fukumitsu, Ken Kamo, Naoko Fujita, Naoya Ikai, Iwao Uemoto, Shinji In Vitro Cell Dev Biol Anim Article Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f(+)CD45(−)Thy1(−) cell fraction. However, these cells were a heterogeneous cell population containing various stages of differentiation. This study aimed to detect more immature HPCs, using a novel surface antigen, gp38. After the collagenase digestion of fetal livers harvested from E13.5 to E18.5 fetal mice, HPCs were obtained and divided into two subpopulations using flow cytometry: gp38-positive HPCs, and gp38-negative HPCs. Both types of HPCs were characterized by immunocytochemistry and RT-PCR. The proliferative activity was compared by BrdU incorporation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) assay. Furthermore, the comprehensive gene expression was investigated by DNA microarray. Both types of HPCs expressed alpha-fetoprotein. However, the gp38-positive HPCs derived from E13.5 fetal livers did not express albumin or cytokeratin 19, while the gp38-negative HPCs did. DNA microarray revealed that some genes related to the Wnt signal pathway were up-regulated in the gp38-positive HPCs. Furthermore, Wnt3a had a proliferative effect on the gp38-positive HPCs. In conclusion, the gp38-positive HPCs derived from fetal liver tissue until E13.5 could therefore be candidates for hepatic stem cells in the fetal liver. Springer-Verlag 2010-11-20 2011 /pmc/articles/PMC3026936/ /pubmed/21104040 http://dx.doi.org/10.1007/s11626-010-9354-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Konishi, Sayuri
Yasuchika, Kentaro
Ishii, Takamichi
Fukumitsu, Ken
Kamo, Naoko
Fujita, Naoya
Ikai, Iwao
Uemoto, Shinji
A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title_full A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title_fullStr A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title_full_unstemmed A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title_short A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
title_sort transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026936/
https://www.ncbi.nlm.nih.gov/pubmed/21104040
http://dx.doi.org/10.1007/s11626-010-9354-7
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