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Remote focusing for programmable multi-layer differential multiphoton microscopy

We present the application of remote focusing to multiphoton laser scanning microscopy and utilize this technology to demonstrate simultaneous, programmable multi-layer imaging. Remote focusing is used to independently control the axial location of multiple focal planes that can be simultaneously im...

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Detalles Bibliográficos
Autores principales: Hoover, Erich E., Young, Michael D., Chandler, Eric V., Luo, Anding, Field, Jeffrey J., Sheetz, Kraig E., Sylvester, Anne W., Squier, Jeff A.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3028486/
https://www.ncbi.nlm.nih.gov/pubmed/21326641
http://dx.doi.org/10.1364/BOE.1.000113
Descripción
Sumario:We present the application of remote focusing to multiphoton laser scanning microscopy and utilize this technology to demonstrate simultaneous, programmable multi-layer imaging. Remote focusing is used to independently control the axial location of multiple focal planes that can be simultaneously imaged with single element detection. This facilitates volumetric multiphoton imaging in scattering specimens and can be practically scaled to a large number of focal planes. Further, it is demonstrated that the remote focusing control can be synchronized with the lateral scan directions, enabling imaging in orthogonal scan planes.