Two-photon excited hemoglobin fluorescence

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Sore...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Wei, Li, Dong, Zeng, Yan, Luo, Yi, Qu, Jianan Y.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Spectroscopic Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3028500/
https://www.ncbi.nlm.nih.gov/pubmed/21326637
http://dx.doi.org/10.1364/BOE.2.000071
_version_ 1782197179477131264
author Zheng, Wei
Li, Dong
Zeng, Yan
Luo, Yi
Qu, Jianan Y.
author_facet Zheng, Wei
Li, Dong
Zeng, Yan
Luo, Yi
Qu, Jianan Y.
author_sort Zheng, Wei
collection PubMed
description We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.
format Text
id pubmed-3028500
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Optical Society of America
record_format MEDLINE/PubMed
spelling pubmed-30285002011-02-16 Two-photon excited hemoglobin fluorescence Zheng, Wei Li, Dong Zeng, Yan Luo, Yi Qu, Jianan Y. Biomed Opt Express Spectroscopic Diagnostics We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels. Optical Society of America 2010-12-06 /pmc/articles/PMC3028500/ /pubmed/21326637 http://dx.doi.org/10.1364/BOE.2.000071 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Spectroscopic Diagnostics
Zheng, Wei
Li, Dong
Zeng, Yan
Luo, Yi
Qu, Jianan Y.
Two-photon excited hemoglobin fluorescence
title Two-photon excited hemoglobin fluorescence
title_full Two-photon excited hemoglobin fluorescence
title_fullStr Two-photon excited hemoglobin fluorescence
title_full_unstemmed Two-photon excited hemoglobin fluorescence
title_short Two-photon excited hemoglobin fluorescence
title_sort two-photon excited hemoglobin fluorescence
topic Spectroscopic Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3028500/
https://www.ncbi.nlm.nih.gov/pubmed/21326637
http://dx.doi.org/10.1364/BOE.2.000071
work_keys_str_mv AT zhengwei twophotonexcitedhemoglobinfluorescence
AT lidong twophotonexcitedhemoglobinfluorescence
AT zengyan twophotonexcitedhemoglobinfluorescence
AT luoyi twophotonexcitedhemoglobinfluorescence
AT qujianany twophotonexcitedhemoglobinfluorescence

Ejemplares similares