Cargando…
Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition
Nuclear migration is regulated by the LIS1 protein, which is the regulatory subunit of platelet activating factor (PAF) acetyl-hydrolase, an enzyme complex that inactivates the lipid mediator PAF. Among other functions, PAF modulates cell proliferation, but its effects upon mechanisms of the cell cy...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3029264/ https://www.ncbi.nlm.nih.gov/pubmed/21298035 http://dx.doi.org/10.1371/journal.pone.0016058 |
_version_ | 1782197212532441088 |
---|---|
author | Fragel-Madeira, Lucianne Meletti, Tamara Mariante, Rafael M. Monteiro, Robson Q. Einicker-Lamas, Marcelo Bernardo, Robson R. Lopes, Angela H. Linden, Rafael |
author_facet | Fragel-Madeira, Lucianne Meletti, Tamara Mariante, Rafael M. Monteiro, Robson Q. Einicker-Lamas, Marcelo Bernardo, Robson R. Lopes, Angela H. Linden, Rafael |
author_sort | Fragel-Madeira, Lucianne |
collection | PubMed |
description | Nuclear migration is regulated by the LIS1 protein, which is the regulatory subunit of platelet activating factor (PAF) acetyl-hydrolase, an enzyme complex that inactivates the lipid mediator PAF. Among other functions, PAF modulates cell proliferation, but its effects upon mechanisms of the cell cycle are unknown. Here we show that PAF inhibited interkinetic nuclear migration (IKNM) in retinal proliferating progenitors. The lipid did not, however, affect the velocity of nuclear migration in cells that escaped IKNM blockade. The effect depended on the PAF receptor, Erk and p38 pathways and Chk1. PAF induced no cell death, nor a reduction in nucleotide incorporation, which rules out an intra-S checkpoint. Notwithstanding, the expected increase in cyclin B1 content during G2-phase was prevented in the proliferating cells. We conclude that PAF blocks interkinetic nuclear migration in retinal progenitor cells through an unusual arrest of the cell cycle at the transition from S to G2 phases. These data suggest the operation, in the developing retina, of a checkpoint that monitors the transition from S to G2 phases of the cell cycle. |
format | Text |
id | pubmed-3029264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30292642011-02-04 Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition Fragel-Madeira, Lucianne Meletti, Tamara Mariante, Rafael M. Monteiro, Robson Q. Einicker-Lamas, Marcelo Bernardo, Robson R. Lopes, Angela H. Linden, Rafael PLoS One Research Article Nuclear migration is regulated by the LIS1 protein, which is the regulatory subunit of platelet activating factor (PAF) acetyl-hydrolase, an enzyme complex that inactivates the lipid mediator PAF. Among other functions, PAF modulates cell proliferation, but its effects upon mechanisms of the cell cycle are unknown. Here we show that PAF inhibited interkinetic nuclear migration (IKNM) in retinal proliferating progenitors. The lipid did not, however, affect the velocity of nuclear migration in cells that escaped IKNM blockade. The effect depended on the PAF receptor, Erk and p38 pathways and Chk1. PAF induced no cell death, nor a reduction in nucleotide incorporation, which rules out an intra-S checkpoint. Notwithstanding, the expected increase in cyclin B1 content during G2-phase was prevented in the proliferating cells. We conclude that PAF blocks interkinetic nuclear migration in retinal progenitor cells through an unusual arrest of the cell cycle at the transition from S to G2 phases. These data suggest the operation, in the developing retina, of a checkpoint that monitors the transition from S to G2 phases of the cell cycle. Public Library of Science 2011-01-27 /pmc/articles/PMC3029264/ /pubmed/21298035 http://dx.doi.org/10.1371/journal.pone.0016058 Text en Fragel-Madeira et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fragel-Madeira, Lucianne Meletti, Tamara Mariante, Rafael M. Monteiro, Robson Q. Einicker-Lamas, Marcelo Bernardo, Robson R. Lopes, Angela H. Linden, Rafael Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title | Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title_full | Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title_fullStr | Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title_full_unstemmed | Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title_short | Platelet Activating Factor Blocks Interkinetic Nuclear Migration in Retinal Progenitors through an Arrest of the Cell Cycle at the S/G2 Transition |
title_sort | platelet activating factor blocks interkinetic nuclear migration in retinal progenitors through an arrest of the cell cycle at the s/g2 transition |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3029264/ https://www.ncbi.nlm.nih.gov/pubmed/21298035 http://dx.doi.org/10.1371/journal.pone.0016058 |
work_keys_str_mv | AT fragelmadeiralucianne plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT melettitamara plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT marianterafaelm plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT monteirorobsonq plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT einickerlamasmarcelo plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT bernardorobsonr plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT lopesangelah plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition AT lindenrafael plateletactivatingfactorblocksinterkineticnuclearmigrationinretinalprogenitorsthroughanarrestofthecellcycleatthesg2transition |