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Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins
The Influenza A virus non-structural protein 1 (NS1) is a multifunctional virulence factor with several protein-protein interaction domains, involved in preventing apoptosis of the infected cell and in evading the interferon response. In addition, the majority of influenza A virus NS1 proteins have...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030508/ https://www.ncbi.nlm.nih.gov/pubmed/21247458 http://dx.doi.org/10.1186/1743-422X-8-25 |
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author | Thomas, Miranda Kranjec, Christian Nagasaka, Kazunori Matlashewski, Greg Banks , Lawrence |
author_facet | Thomas, Miranda Kranjec, Christian Nagasaka, Kazunori Matlashewski, Greg Banks , Lawrence |
author_sort | Thomas, Miranda |
collection | PubMed |
description | The Influenza A virus non-structural protein 1 (NS1) is a multifunctional virulence factor with several protein-protein interaction domains, involved in preventing apoptosis of the infected cell and in evading the interferon response. In addition, the majority of influenza A virus NS1 proteins have a class I PDZ-binding motif at the C-terminus, and this itself has been shown to be a virulence determinant. In the majority of human influenza NS1 proteins the consensus motif is RSxV: in avian NS1 it is ESxV. Of the few human strains that have the avian motif, all were from very high mortality outbreaks of the disease. Previous work has shown that minor differences in PDZ-binding motifs can have major effects on the spectrum of cellular proteins targeted. In this study we analyse the effect of these differences upon the binding of Influenza A virus NS1 protein to a range of cellular proteins involved in polarity and signal transduction. |
format | Text |
id | pubmed-3030508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30305082011-01-29 Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins Thomas, Miranda Kranjec, Christian Nagasaka, Kazunori Matlashewski, Greg Banks , Lawrence Virol J Research The Influenza A virus non-structural protein 1 (NS1) is a multifunctional virulence factor with several protein-protein interaction domains, involved in preventing apoptosis of the infected cell and in evading the interferon response. In addition, the majority of influenza A virus NS1 proteins have a class I PDZ-binding motif at the C-terminus, and this itself has been shown to be a virulence determinant. In the majority of human influenza NS1 proteins the consensus motif is RSxV: in avian NS1 it is ESxV. Of the few human strains that have the avian motif, all were from very high mortality outbreaks of the disease. Previous work has shown that minor differences in PDZ-binding motifs can have major effects on the spectrum of cellular proteins targeted. In this study we analyse the effect of these differences upon the binding of Influenza A virus NS1 protein to a range of cellular proteins involved in polarity and signal transduction. BioMed Central 2011-01-19 /pmc/articles/PMC3030508/ /pubmed/21247458 http://dx.doi.org/10.1186/1743-422X-8-25 Text en Copyright ©2011 Thomas et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Thomas, Miranda Kranjec, Christian Nagasaka, Kazunori Matlashewski, Greg Banks , Lawrence Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title | Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title_full | Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title_fullStr | Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title_full_unstemmed | Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title_short | Analysis of the PDZ binding specificities of Influenza A Virus NS1 proteins |
title_sort | analysis of the pdz binding specificities of influenza a virus ns1 proteins |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030508/ https://www.ncbi.nlm.nih.gov/pubmed/21247458 http://dx.doi.org/10.1186/1743-422X-8-25 |
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