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Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging
BACKGROUND: Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated i...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031243/ https://www.ncbi.nlm.nih.gov/pubmed/21232134 http://dx.doi.org/10.1186/1742-4933-8-3 |
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author | Dowd, Jennifer B Aiello, Allison E Chyu, Laura Huang, Yuan-yen McDade, Thomas W |
author_facet | Dowd, Jennifer B Aiello, Allison E Chyu, Laura Huang, Yuan-yen McDade, Thomas W |
author_sort | Dowd, Jennifer B |
collection | PubMed |
description | BACKGROUND: Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated immunity. RESULTS: Analysis of CMV in dried blood spot samples (DBS) was based on modifications of a commercially available protocol for quantifying CMV antibodies in serum or plasma. The method was evaluated through analysis of precision, reliability, linearity, and correlation between matched serum and DBS samples collected from 75 volunteers. Correlation between DBS and plasma values was linear and high (Pearson correlation R = .96), and precision, reliability, and linearity of the DBS assay were within acceptable ranges. CONCLUSIONS: The validity of a DBS assay for CMV antibodies will enable its inclusion in population-based surveys and other studies collecting DBS samples in non-clinical settings, increasing scientific understanding of the interaction of social and biological stress and immune function. |
format | Text |
id | pubmed-3031243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30312432011-02-01 Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging Dowd, Jennifer B Aiello, Allison E Chyu, Laura Huang, Yuan-yen McDade, Thomas W Immun Ageing Methodology BACKGROUND: Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated immunity. RESULTS: Analysis of CMV in dried blood spot samples (DBS) was based on modifications of a commercially available protocol for quantifying CMV antibodies in serum or plasma. The method was evaluated through analysis of precision, reliability, linearity, and correlation between matched serum and DBS samples collected from 75 volunteers. Correlation between DBS and plasma values was linear and high (Pearson correlation R = .96), and precision, reliability, and linearity of the DBS assay were within acceptable ranges. CONCLUSIONS: The validity of a DBS assay for CMV antibodies will enable its inclusion in population-based surveys and other studies collecting DBS samples in non-clinical settings, increasing scientific understanding of the interaction of social and biological stress and immune function. BioMed Central 2011-01-13 /pmc/articles/PMC3031243/ /pubmed/21232134 http://dx.doi.org/10.1186/1742-4933-8-3 Text en Copyright ©2011 Dowd et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Dowd, Jennifer B Aiello, Allison E Chyu, Laura Huang, Yuan-yen McDade, Thomas W Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title | Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title_full | Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title_fullStr | Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title_full_unstemmed | Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title_short | Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
title_sort | cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031243/ https://www.ncbi.nlm.nih.gov/pubmed/21232134 http://dx.doi.org/10.1186/1742-4933-8-3 |
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