Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG

We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the pred...

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Autores principales: van Eeden, Pauline E., Wiese, Michael D., Aulfrey, Susan, Hales, Belinda J., Stone, Shelley F., Brown, Simon G. A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031629/
https://www.ncbi.nlm.nih.gov/pubmed/21304970
http://dx.doi.org/10.1371/journal.pone.0016741
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author van Eeden, Pauline E.
Wiese, Michael D.
Aulfrey, Susan
Hales, Belinda J.
Stone, Shelley F.
Brown, Simon G. A.
author_facet van Eeden, Pauline E.
Wiese, Michael D.
Aulfrey, Susan
Hales, Belinda J.
Stone, Shelley F.
Brown, Simon G. A.
author_sort van Eeden, Pauline E.
collection PubMed
description We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms.
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spelling pubmed-30316292011-02-08 Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG van Eeden, Pauline E. Wiese, Michael D. Aulfrey, Susan Hales, Belinda J. Stone, Shelley F. Brown, Simon G. A. PLoS One Research Article We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG(1) and sIgG(4) in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intra-assay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms. Public Library of Science 2011-01-31 /pmc/articles/PMC3031629/ /pubmed/21304970 http://dx.doi.org/10.1371/journal.pone.0016741 Text en van Eeden et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
van Eeden, Pauline E.
Wiese, Michael D.
Aulfrey, Susan
Hales, Belinda J.
Stone, Shelley F.
Brown, Simon G. A.
Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title_full Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title_fullStr Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title_full_unstemmed Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title_short Using Time-Resolved Fluorescence to Measure Serum Venom-Specific IgE and IgG
title_sort using time-resolved fluorescence to measure serum venom-specific ige and igg
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031629/
https://www.ncbi.nlm.nih.gov/pubmed/21304970
http://dx.doi.org/10.1371/journal.pone.0016741
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