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Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells

Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells. We propose a Wide-Field Multi-Parameter FLIM method (WFMP-FLIM) aimed to monitor continuously living cells under minimum light intensity...

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Detalles Bibliográficos
Autores principales:	Vitali, Marco, Picazo, Fernando, Prokazov, Yury, Duci, Alessandro, Turbin, Evgeny, Götze, Christian, Llopis, Juan, Hartig, Roland, Visser, Antonie J. W. G., Zuschratter, Werner
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032730/ https://www.ncbi.nlm.nih.gov/pubmed/21311595 http://dx.doi.org/10.1371/journal.pone.0015820

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