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Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP c...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032800/ https://www.ncbi.nlm.nih.gov/pubmed/20729842 http://dx.doi.org/10.1038/nmeth.1493 |
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author | Matov, Alexandre Applegate, Kathryn Kumar, Praveen Thoma, Claudio Krek, Wilhelm Danuser, Gaudenz Wittmann, Torsten |
author_facet | Matov, Alexandre Applegate, Kathryn Kumar, Praveen Thoma, Claudio Krek, Wilhelm Danuser, Gaudenz Wittmann, Torsten |
author_sort | Matov, Alexandre |
collection | PubMed |
description | Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP comets visible in an image time-lapse sequence allowing the detection of spatial patterns of microtubule dynamics. We use spatiotemporal clustering of EB1-EGFP growth tracks to infer microtubule behaviors during phases of pause and shortening. The algorithm was validated by comparison to manually tracked, homogeneously labeled microtubules, and by analysis of the effects of well-characterized inhibitors of microtubule polymerization dynamics. We used our method to analyze spatial variations of intracellular microtubule dynamics in migrating epithelial cells. |
format | Text |
id | pubmed-3032800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-30328002011-03-01 Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker Matov, Alexandre Applegate, Kathryn Kumar, Praveen Thoma, Claudio Krek, Wilhelm Danuser, Gaudenz Wittmann, Torsten Nat Methods Article Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP comets visible in an image time-lapse sequence allowing the detection of spatial patterns of microtubule dynamics. We use spatiotemporal clustering of EB1-EGFP growth tracks to infer microtubule behaviors during phases of pause and shortening. The algorithm was validated by comparison to manually tracked, homogeneously labeled microtubules, and by analysis of the effects of well-characterized inhibitors of microtubule polymerization dynamics. We used our method to analyze spatial variations of intracellular microtubule dynamics in migrating epithelial cells. 2010-08-22 2010-09 /pmc/articles/PMC3032800/ /pubmed/20729842 http://dx.doi.org/10.1038/nmeth.1493 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Matov, Alexandre Applegate, Kathryn Kumar, Praveen Thoma, Claudio Krek, Wilhelm Danuser, Gaudenz Wittmann, Torsten Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title | Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title_full | Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title_fullStr | Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title_full_unstemmed | Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title_short | Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker |
title_sort | analysis of microtubule dynamic instability using a plus end growth marker |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032800/ https://www.ncbi.nlm.nih.gov/pubmed/20729842 http://dx.doi.org/10.1038/nmeth.1493 |
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