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Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker

Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP c...

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Detalles Bibliográficos
Autores principales: Matov, Alexandre, Applegate, Kathryn, Kumar, Praveen, Thoma, Claudio, Krek, Wilhelm, Danuser, Gaudenz, Wittmann, Torsten
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032800/
https://www.ncbi.nlm.nih.gov/pubmed/20729842
http://dx.doi.org/10.1038/nmeth.1493
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author Matov, Alexandre
Applegate, Kathryn
Kumar, Praveen
Thoma, Claudio
Krek, Wilhelm
Danuser, Gaudenz
Wittmann, Torsten
author_facet Matov, Alexandre
Applegate, Kathryn
Kumar, Praveen
Thoma, Claudio
Krek, Wilhelm
Danuser, Gaudenz
Wittmann, Torsten
author_sort Matov, Alexandre
collection PubMed
description Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP comets visible in an image time-lapse sequence allowing the detection of spatial patterns of microtubule dynamics. We use spatiotemporal clustering of EB1-EGFP growth tracks to infer microtubule behaviors during phases of pause and shortening. The algorithm was validated by comparison to manually tracked, homogeneously labeled microtubules, and by analysis of the effects of well-characterized inhibitors of microtubule polymerization dynamics. We used our method to analyze spatial variations of intracellular microtubule dynamics in migrating epithelial cells.
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spelling pubmed-30328002011-03-01 Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker Matov, Alexandre Applegate, Kathryn Kumar, Praveen Thoma, Claudio Krek, Wilhelm Danuser, Gaudenz Wittmann, Torsten Nat Methods Article Regulation of microtubule dynamics is essential for many cell biological processes, and is likely to be variable between different subcellular regions. We describe a computational approach to analyze microtubule dynamics by detecting growing microtubule plus ends. Our algorithm tracks all EB1-EGFP comets visible in an image time-lapse sequence allowing the detection of spatial patterns of microtubule dynamics. We use spatiotemporal clustering of EB1-EGFP growth tracks to infer microtubule behaviors during phases of pause and shortening. The algorithm was validated by comparison to manually tracked, homogeneously labeled microtubules, and by analysis of the effects of well-characterized inhibitors of microtubule polymerization dynamics. We used our method to analyze spatial variations of intracellular microtubule dynamics in migrating epithelial cells. 2010-08-22 2010-09 /pmc/articles/PMC3032800/ /pubmed/20729842 http://dx.doi.org/10.1038/nmeth.1493 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Matov, Alexandre
Applegate, Kathryn
Kumar, Praveen
Thoma, Claudio
Krek, Wilhelm
Danuser, Gaudenz
Wittmann, Torsten
Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title_full Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title_fullStr Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title_full_unstemmed Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title_short Analysis of Microtubule Dynamic Instability Using a Plus End Growth Marker
title_sort analysis of microtubule dynamic instability using a plus end growth marker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032800/
https://www.ncbi.nlm.nih.gov/pubmed/20729842
http://dx.doi.org/10.1038/nmeth.1493
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