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Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario
The Walkerton, Canada, waterborne outbreak of 2000 resulted from entry of Escherichia coli O157:H7 and Campylobacter spp. from neighboring farms into the town water supply. Isolates of Campylobacter jejuni and Campylobacter coli obtained from outbreak investigations were characterized by phenotypic...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Centers for Disease Control and Prevention
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033067/ https://www.ncbi.nlm.nih.gov/pubmed/14609457 http://dx.doi.org/10.3201/eid0910.020584 |
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author | Clark, Clifford G. Price, Lawrence Ahmed, Rafiq Woodward, David L. Melito, Pasquale L. Rodgers, Frank G. Jamieson, Frances Ciebin, Bruce Li, Aimin Ellis, Andrea |
author_facet | Clark, Clifford G. Price, Lawrence Ahmed, Rafiq Woodward, David L. Melito, Pasquale L. Rodgers, Frank G. Jamieson, Frances Ciebin, Bruce Li, Aimin Ellis, Andrea |
author_sort | Clark, Clifford G. |
collection | PubMed |
description | The Walkerton, Canada, waterborne outbreak of 2000 resulted from entry of Escherichia coli O157:H7 and Campylobacter spp. from neighboring farms into the town water supply. Isolates of Campylobacter jejuni and Campylobacter coli obtained from outbreak investigations were characterized by phenotypic and genotypic methods, including heat-stable and heat-labile serotyping, phage typing, biotyping, fla–restriction fragment length polymorphism (RFLP) typing, and pulsed-field gel electrophoresis. Two main outbreak strains were identified on the basis of heat-stable serotyping and fla-RFLP typing. These strains produced a limited number of types when tested by other methods. Isolates with types indistinguishable from, or similar to, the outbreak types were found only on one farm near the town of Walkerton, whereas cattle from other farms carried a variety of Campylobacter strains with different type characteristics. Results of these analyses confirmed results from epidemiologic studies and the utility of using several different typing and subtyping methods for completely characterizing bacterial populations. |
format | Text |
id | pubmed-3033067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-30330672011-02-09 Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario Clark, Clifford G. Price, Lawrence Ahmed, Rafiq Woodward, David L. Melito, Pasquale L. Rodgers, Frank G. Jamieson, Frances Ciebin, Bruce Li, Aimin Ellis, Andrea Emerg Infect Dis Research The Walkerton, Canada, waterborne outbreak of 2000 resulted from entry of Escherichia coli O157:H7 and Campylobacter spp. from neighboring farms into the town water supply. Isolates of Campylobacter jejuni and Campylobacter coli obtained from outbreak investigations were characterized by phenotypic and genotypic methods, including heat-stable and heat-labile serotyping, phage typing, biotyping, fla–restriction fragment length polymorphism (RFLP) typing, and pulsed-field gel electrophoresis. Two main outbreak strains were identified on the basis of heat-stable serotyping and fla-RFLP typing. These strains produced a limited number of types when tested by other methods. Isolates with types indistinguishable from, or similar to, the outbreak types were found only on one farm near the town of Walkerton, whereas cattle from other farms carried a variety of Campylobacter strains with different type characteristics. Results of these analyses confirmed results from epidemiologic studies and the utility of using several different typing and subtyping methods for completely characterizing bacterial populations. Centers for Disease Control and Prevention 2003-10 /pmc/articles/PMC3033067/ /pubmed/14609457 http://dx.doi.org/10.3201/eid0910.020584 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Clark, Clifford G. Price, Lawrence Ahmed, Rafiq Woodward, David L. Melito, Pasquale L. Rodgers, Frank G. Jamieson, Frances Ciebin, Bruce Li, Aimin Ellis, Andrea Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title | Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title_full | Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title_fullStr | Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title_full_unstemmed | Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title_short | Characterization of Waterborne Outbreak–associated Campylobacter jejuni, Walkerton, Ontario |
title_sort | characterization of waterborne outbreak–associated campylobacter jejuni, walkerton, ontario |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3033067/ https://www.ncbi.nlm.nih.gov/pubmed/14609457 http://dx.doi.org/10.3201/eid0910.020584 |
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